| 货号 | 2307T |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | p38γ MAPK Antibody detects endogenous levels of total p38γ MAPK protein. This antibody does not cross-react with the other p38 MAPK isoforms p38α, -β or -δ. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAP kinase, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAP kinase by phosphorylation at Thr180 and Tyr182. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8). SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10). |
| 存放说明 | -20C |
| 计算分子量 | 46 |
| 参考文献 | 1 . Rouse, J. et al. (1994) Cell 78, 1027-1037. 2 . Han, J. et al. (1994) Science 265, 808-11. 3 . Lee, J.C. et al. (1994) Nature 372, 739-46. 4 . Freshney, N.W. et al. (1994) Cell 78, 1039-49. 5 . Raingeaud, J. et al. (1995) J. Biol. Chem. 270, 7420-7426. 6 . Zervos, A.S. et al. (1995) Proc Natl Acad Sci U S A 92, 10531-4. 7 . Zhao, M. et al. (1999) Mol Cell Biol 19, 21-30. 8 . Yang, S.H. et al. (1999) Mol Cell Biol 19, 4028-38. 9 . Cuenda, A. et al. (1995) FEBS Lett 364, 229-33. 10 . Kumar, S. et al. (1999) Biochem Biophys Res Commun 263, 825-31. 11 . Fearns, C. et al. (2000) J. Leukoc. Biol. 67, 705-711. 12 . Hale, K.K. et al. (1999) J. Immunol. 162, 4246-4252. |
Western blot analysis of purified recombinant full-length p38 MAPK GST fusion protein, using p38 MAP kinase pan antibody (upper) or p38γ MAPK Antibody (lower).Western blot 检测纯化的重组全长p38 MAPK GST融合蛋白，使用的抗体是p38 MAP kinase pan antibody (上图)和p38γ MAPK Antibody (下图)。 | |
Western blot analysis of extracts from HUVEC and U937 cells, using p38γ MAPK Antibody.Western blot 检测总细胞提取物：HUVEC、U937细胞，使用的抗体是p38γ MAPK Antibody。 |
