| 货号 | 2729S |
| 描述 | Normal Rabbit IgG is an unconjugated rabbit polyclonal antibody that is routinely used as a non-specific IgG control in chromatin immunoprecipitation using our SimpleChIP® Enzymatic Chromatin IP Kits #9002 and #9003. |
| 来源宿主 | Rabbit |
| 应用 | IP/ChIP |
| 使用方法 | ChIP () |
| 供应商 | CST |
| 背景 | Normal Rabbit IgG is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody. |
| 存放说明 | -20C |
Chromatin immunoprecipitations were performed using digested chromatin from HeLa cells and the indicated antibodies. Purified DNA was analyzed by quantitative real-time PCR, using SimpleChIP® Human RPL30 Exon 3 Primers #7014, SimpleChIP® Human MyoD1 Exon 1 Primers #4490, and SimpleChIP® Human α Satellite Repeat Primers #4486. The relative abundance of each DNA sequence enriched by Normal Rabbit IgG (red) is compared to the amount of the same DNA sequence enriched by the protein-specific immunoprecipitations.采用HeLa细胞已消化的染色质和已知抗体进行染色质免疫沉淀。实时定量PCR检测纯化DNA，所用引物分别为SimpleChIP ® Human RPL30 Exon 3 Primers #7014，SimpleChIP™ Human MyoD1 Exon 1 Primers #4490和 SimpleChIP™ Human α Satellite Repeat Primers #4486。每一条DNA序列的相对含量，所用抗体为Normal Rabbit IgG (红色) 与同一条DNA序列的含量做比较，后者通过为蛋白特异的免疫沉淀检测。 | |
Chromatin immunoprecipitations were performed using digested chromatin from HeLa cells and either Histone H3 (D2B12) XP® Rabbit mAb (ChIP Formulated) #4620 (lane 2), Rpb1 CTD (4H8) Mouse mAb #2629 (lane 3), Di-Methyl Histone H3 (Lys9) Antibody #9753 (lane 4) or Normal Rabbit IgG #2729 (lane 5). Purified DNA was analyzed by standard PCR methods using SimpleChIP® Human RPL30 Exon 3 Primers #7014, SimpleChIP® Human MyoD1 Exon 1 Primers #4490, and SimpleChIP® Human a Satellite Repeat Primers #4486. PCR products were observed for each primer set in the input sample (lane 1) and various protein-specific immunoprecipitations but no PCR products were observed with immunoprecipitation using Normal Rabbit IgG #2729 (lane 5).采用HeLa细胞已消化的染色质和已知抗体进行染色质免疫沉淀。所用抗体为Histone H3 (D2B12) XP™ Rabbit mAb (ChIP Formulated) #4620 (泳道 2), Rpb1 CTD (4H8) Mouse mAb #2629 (泳道 3), Di-Methyl Histone H3 (Lys9) Antibody #9753 (泳道 4) or Normal Rabbit IgG #2729 (泳道 5)。实时定量PCR检测纯化DNA，所用引物特异性针对e RPL30基因、 MyoD1 基因或异染色质α卫星重复元件。针对每一对引物的PCR产物都可以被观察到（泳道1），各种蛋白特异的免疫沉淀用Normal Rabbit IgG #2729 都可以被观察到，但PCR产物则没有（泳道5）。 |
