| 货号 | MAB3548-SP |
| 别名 | Fasciclin I-like; MGC119510; MGC119511; OSF-2; OSF-2osteoblast specific factor 2 (fasciclin I-like); OSF2periodontal ligament-specific periostin; Osteoblast-specific factor 2; PDLPOSTN; periostin isoform thy2; periostin isoform thy4; periostin isoform thy6; periostin isoform thy8; periostin; periostin, osteoblast specific factor; PNRP11-412K4.1; POSTN; TRIF52 | 全称 | Osteoblast Specific Factor 2 |
| 反应种属 | Mouse |
| 应用 | Western Blot(1 µg/mL) Immunocytochemistry(8-25 µg/mL) |
| 目标/特异性 | Detects mouse Periostin/OSF-2 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunocytochemistry: 8-25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10631 (Human); 50706 (Mouse); 361945 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Beta3 integrin modulates transforming growth factor beta induced (TGFBI) function and paclitaxel response in ovarian cancer cells. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Periostin isoform 2 Asn24-Gln811 Accession # NP_056599 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Mouse periostin, also known as OSF-2 (osteoblast-specific factor 2) is a 170 kDa, secreted, homodimeric protein that belongs to the periostin family of the FAS1 superfamily of molecules (1-4). It is a TGF-beta inducible molecule that serves as both an adhesion molecule and tumor suppressor (2, 5, 6, 7). It is synthesized as a 838 amino acid (aa) precursor that contains a 23 aa signal sequence and an 815 aa mature region (2, 8). It is unknown if the molecule has any significant glycosylation (2). Based on human OSF-2, the homodimer is not disulfide-linked (3). The molecule consists of two distinct regions. The N-terminus contains an 55 aa EMI domain, while theC-terminus contains four, 130 aa fasciculin type 1 (or FAS1) domains. The EMI domain is cysteine-rich and shows a highly basic alpha -helix (9). Each FAS1 repeat exhibits a novel 7-stranded beta -wedge with a multiple alpha -helix fold (1, 8). Multiple alternate splice forms are known to exist C-terminal (aa 672-812) to the four-fold FAS1 repeats. These mature molecules are 760, 761, 787, and 788 aa in length and show block deletions of 54 aa, 27 aa and/or 28 aa (10). The significance of the alternate splice forms is not clear. They do, however, appear to be temporally regulated (6). OSF-2 is known to bind to alpha vbeta3 and alpha vbeta5 integrins (3). It is synthesized by smooth muscle cells, fibroblasts, and osteoblasts (2, 5, 7). Mature mouse OSF-2 is 98%, 92%, and 91% aa identical to rat, canine, and human OSF-2, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Periostin/OSF‑2 in Human Osteocytes. Periostin/OSF-2 was detected in immersion fixed human osteocytes using Rat Anti-Human/Mouse Periostin/OSF-2 Monoclonal Antibody (Catalog # MAB3548) at 10 µg/mL for 3 hours at room temperature. Cells were stained red and counterstained with DAPI (blue). |
