Human Cadherin-12 MAb (Clone 343621) (25 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human Cadherin-12 MAb (Clone 343621) (25 UG)

货号： MAB2240-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB2240-SP
别名	Br-Cadherin; cadherin 12, type 2 (N-cadherin 2); cadherin-12; CDH12; CDHB; FLJ34857; N-Cadherin 2; Neural type cadherin 2; neural type, 2; neuronal cadherin 2
反应种属	Human
应用	Western Blot(1 µg/mL) Flow Cytometry(0.25 µg/10⁶cells) Immunocytochemistry(8-25 µg/mL)
目标/特异性	Detects human Cadherin‑12 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Cadherin-8, -11, -13, -17, rhE-Cadherin, rhP-Cadherin, rhR-Cadherin, or rhVR-Cadherin is observed.
使用方法	Western Blot: 1 µg/mL Flow Cytometry: 0.25 µg/10⁶cells Immunocytochemistry: 8-25 µg/mL
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	1010 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. N-cadherin is regulated by activin A and associated with tumor aggressiveness in esophageal carcinoma. Authors: Utsunomiya T, Sonoda H, Mimori K Clin. Cancer Res., 2004;10(17):5702-7. Species: Human Sample Type: Tissue Homogenates Application: WB
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Cadherin-12 Met1-Pro609 Accession # P55289
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	The cadherin superfamily is a large family of membrane-associated glycoproteins that engage in homotypic, calcium-dependent, cell-cell adhesion events. The superfamily can be divided into at least four subfamilies based on its member’s extracellular (EC) regions and cytoplasmic domains (1, 2). These include classical cadherins, desmosomal cadherins, protocadherins, and cadherin-like molecules that contain a variable number of EC and transmembrane (TM) domains (1). Cadherin‑12, also known as brain-cadherin and N-cadherin 2, is a 150 kDa classical cadherin. Classical family molecules are modular in their extracellular region, mediating calcium-dependent cell-cell adhesion through their five EC Ca⁺⁺-binding repeats (2). Cadherin-12 can be further identified as a type II classical cadherin, due to the absence of a His-Ala-Val motif in its most N-terminal cadherin repeat (3). Human Cadherin-12 is synthesized as a 794 amino acid (aa) type I transmembrane preproprotein that contains a 23 aa signal peptide, a 31 aa prosequence, a 555 aa extracellular region, a 28 aa transmembrane segment, and a 157 aa cytoplasmic domain (4, 5). The five EC cadherin domains are approximately 110 aa in length and generate two beta -sheets that are oriented like bread in a sandwich. Human Cadherin-12 EC region is 96% aa identical to mouse Cadherin-12 EC region. Cadherin-12 is expressed specifically in CNS neurons. The bulk of its expression is postnatal, and it is proposed to be involved in synaptogenesis (4). As a classic cadherin, Cadherin-12 will form homodimers and promote intercellular adhesion with itself and, possibly, cadherins-8 and -14 (6).
运输条件	Blue Ice
存放说明	4℃
参考文献	Koch, A.W. et al. (2004) Cell. Mol. Life Sci. 61:1884. Angst, B.D. et al. (2001) J. Cell Sci. 114:629. Gessner, R. and R. Tauber (2000) Ann. N.Y. Acad. Sci. 915:136. Selig, S. et al. (1997) Proc. Natl. Acad. Sci. USA 94:2398. Tanihara, H. et al. (1994) Cell Adhes. Commun. 2:15. Shimoyama, Y. et al. (2000) Biochem. J. 349:159.

参考图片

Cadherin‑12 in BG01V Human Embryonic Stem Cells. Cadherin‑12 was detected in immersion fixed BG01V human embryonic stem cells differentiated into neurons using Rat Anti-Human Cadherin‑12 Monoclonal Antibody (Catalog # MAB2240) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell membranes. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.