| 货号 | MAB3656-SP |
| 别名 | claudin 6; claudin-6; CLDN6; Skullin 2; skullin |
| 反应种属 | Human |
| 应用 | Flow Cytometry(2.5 µg/106cells) Immunocytochemistry(8-25 µg/mL) |
| 目标/特异性 | Stains human Claudin-6 transfected cells but not irrelevant transfectants. |
| 使用方法 | Flow Cytometry: 2.5 µg/106cells Immunocytochemistry: 8-25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 9074 (Human); 54419 (Mouse); 287098 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | NS0 mouse myeloma cell line transfected with human Claudin-6 Met1-Val220 Accession # P56747 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Claudin-6 is a multipass transmembrane protein in the Claudin family.Claudin-6 is expressed by epithelial cells where it participates intissue development and the maintenance of tight junction integrity.Human Claudin-6 shares 88% and 86% amino acid sequence identity withmouse and rat Claudin-6, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Claudin‑6 in iPS2 Human Induced Pluripotent Stem Cells. Claudin‑6 was detected in immersion fixed iPS2 human induced pluripotent stem cells, differentiated into definitive endoderm with StemXVivo Endoderm Kit (Catalog # SC019), using Mouse Anti-Human Claudin‑6 Monoclonal Antibody (Catalog # MAB3656) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). SOX17 was also detected using Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (green; Catalog # AF1924). Specific staining of Claudin-6 was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips. |
