| 货号 | MAB34691-SP |
| 别名 | FLJ14301; protein Wnt-10a; SSPS; wingless-type MMTV integration site family, member 10A | 全称 | Wingless-type MMTV Integration Site Family Member 10A |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(2 µg/mL) |
| 目标/特异性 | Detects mouse and human Wnt-10a in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinantmouse Wnt-1, -2, -2b, -3a, -4, -5a, -5b, recombinant human (rh) Wnt-6 orrhWnt-7a is observed. |
| 使用方法 | Western Blot: 2 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.5 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 80326 (Human); 22409 (Mouse); 316527 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant mouse Wnt-10a Leu55-Glu95 and Gln278-Arg360 Accession # P70701 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.5 mg/mL. |
| 背景 | Wnt-10a is a presumably secreted, glycosylated member of the Wnt family of developmental proteins. Mouse Wnt-10a is synthesized as a 417 amino acid (aa) precursor that contains a 382 aa mature region. The mature region contains 24 cysteines and two potential N-linked glycosylation sites. Mature mouse Wnt-10a shares 96% and 99% aa sequence identity with mature human and rat Wnt-10a, respectively. Wnt-10a shares 64% aa sequence identity with Wnt-10b/12. Wnt-10a is produced by tumors and thymic epithelium. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human and Mouse Wnt‑10a by Western Blot. Western blot shows lysates of Raji human Burkitts lymphoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Wnt‑10a Monoclonal Antibody (Catalog # MAB34691) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Wnt‑10a at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
