| 货号 | MAB33671-SP |
| 别名 | protein Wnt-8b; wingless-type MMTV integration site family, member 8B | 全称 | Wingless-type MMTV Integration Site Family, Member 8b |
| 反应种属 | Mouse |
| 应用 | Western Blot(5 µg/mL) |
| 目标/特异性 | Detects mouse Wnt-8b in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Wnt-2, -7a, -7b, -9a, recombinant mouse Wnt-1, -3a, -4, -5a, -5b, -8a, or -9b is observed. |
| 使用方法 | Western Blot: 5 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7479 (Human); 22423 (Mouse); 293990 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant mouse Wnt‑8b Trp22-Glu52, Leu195-Arg269 Accession # Q9WUD6 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Mouse Wnt-8b is a 40-50 kDa secreted glycoprotein member of the Wnt family of proteins. Mature mouse Wnt-8b is 329 aa in length and contains 24 cysteines plus three potential N-linked glycosylation sites. Little is known about Wnt-8b. It appears to play a role in forebrain development. The mature mouse protein is 100%, 98%, and 97% identical to mature rat, canine, and human Wnt-8b, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse Wnt‑8b by Western Blot. Western blot shows lysates of KATO-III human gastric carcinoma cell line. PVDF membrane was probed with 5 µg/mL Rat Anti-Mouse Wnt‑8b Monoclonal Antibody (Catalog # MAB33671) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, recombinant mouse Wnt-8b (20 ng) was included. A specific band for native Wnt-8b was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6. |
