| 货号 | MAB3206-SP |
| 别名 | EC 2.7.10; EC 2.7.10.2; FLJ26625; Hck-2; JTK8; tyrosine-protein kinase Lyn; v-yes-1 Yamaguchi sarcoma viral related oncogene homolog; v-yes-1; Yamaguchi sarcoma viral (v-yes-1) related oncogene homolog | 全称 | V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog |
| 反应种属 | Human/Rat |
| 应用 | Western Blot(0.2 µg/mL) Immunocytochemistry(8-25 µg/mL) |
| 目标/特异性 | Detects human and rat Lyn. |
| 使用方法 | Western Blot: 0.2 µg/mL Immunocytochemistry: 8-25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4067 (Human); 17096 (Mouse); 81515 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human Lyn Met1-Ile67 Accession # P07948 |
| 生物活性 | Human, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | The nonreceptor tyrosine kinase Lyn (lck/yes-related novel tyrosine kinase) is widely expressed, and the predominant Src family member present in B cells. Activation of Lyn occurs upon its association with cell surface receptors such as BCR and CD40. Analyses from both loss- and gain-of-function mutant mice have revealed that Lyn is an essential regulator of B cell activation, maturation, and tolerance. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human/Rat Lyn by Western Blot. Western blot shows lysates of Ramos human Burkitts lymphoma cell line and Raji human Burkitts lymphoma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Rat Lyn Monoclonal Antibody (Catalog # MAB3206) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Lyn at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Lyn in Raji Human Cell Line. Lyn was detected in immersion fixed Raji human Burkitts lymphoma cell line using Mouse Anti-Human/Rat Lyn Monoclonal Antibody (Catalog # MAB3206) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
