| 货号 | MAB30701-SP |
| 别名 | IMD2THC; Thrombocytopenia 1; WAS; WASP; WiskTHC1; wiskthrombocytopenia 1 (X-linked) | 全称 | Wiskott-Aldrich Syndrome Protein |
| 反应种属 | Human |
| 应用 | Western Blot(2.5 µg/mL) |
| 目标/特异性 | Detects human WASP in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 2.5 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7454 (Human); 22376 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human WASP Lys230-Gln310 (predicted) Accession # P42768 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | WASP (Wiskott-Aldrich Syndrome Protein) is a 54 kDa protein belonging to the WASP/SCAR/WAVE family of proteins involved in actin polymerization. It is a highly conserved protein and is expressed selectively in hematopoietic stem cell-derived lineages. WASP contains multiple domains with unique functions. Mutations in WASP are associated with diseases of the hematopoietic/ immunologic system. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human WASP by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2.5 µg/mL of Mouse Anti-Human WASP Monoclonal Antibody (Catalog # MAB30701) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for WASP at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
