| 货号 | MAB1648-SP |
| 别名 | CDC25A2; CDC25A2-CAG isoform; cell division cycle 25 homolog A (S. cerevisiae); cell division cycle 25 homolog A (S. pombe); cell division cycle 25A; Dual specificity phosphatase Cdc25A; EC 3.1.3.48; M-phase inducer phosphatase 1 | 全称 | Cell Division Cycle 25A |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(8-25 µg/mL) |
| 目标/特异性 | Detects human, mouse, and rat CDC25A in direct ELISAs and Western blots. In direct ELISA and Western blots, this antibody does not cross-react with recombinant human (rh) CDC25B or rhCDC25C. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 8-25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 993 (Human) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Aurora kinase A is a target of Wnt/beta-catenin involved in multiple myeloma disease progression. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human CDC25A Glu2-Leu523 Accession # AAA58415 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Cell Division Cycle 25A (CDC25A) phosphatase removes inorganic phosphate groups covalently attached to tyrosine, serine and threonine residues in proteins (1). Overexpression of CDC25A in small mammary carcinomas has been associated with a poor patient survival prognosis (2). Levels of CDC25A activity are highest in S phase of the cell cycle, where it is suspected to be involved in “checkpoint” control of cell cycle progression (3). Induction of DNA damage with ultraviolet or ionizing radiation causes an increase in CDC25A ubiquinylation and proteosomal degradation, leading to cell cycle block between G1 and S phases (4). One of its major substrates, the kinase CDC2, is activated by dephosphorylation (5). Recombinant CDC25A is truncated to remove the N-terminal regulatory domains and is fully active. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human/Mouse/Rat CDC25A by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line, CTLL‑2 mouse cytotoxic T cell line, and Nb2‑11 rat lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat CDC25A Monoclonal Antibody (Catalog # MAB1648) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for CDC25A at approximately 55-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
