Mouse S100A8 MAb (Clone 335806) (25 UG)【科瑞奥博】

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Mouse S100A8 MAb (Clone 335806) (25 UG)

货号： MAB3059-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB3059-SP
别名	60B8AG; CAGACP-10; Calgranulin A; calgranulin-A; Calprotectin L1L subunit; CFAG; CFAGL1Ag; CGLA; Cystic fibrosis antigen; Leukocyte L1 complex light chain; MA387; MIF; Migration inhibitory factor-related protein 8; MRP8; MRP-8; MRP8S100 calcium binding protein A8 (calgranulin A); NIF; P8; protein S100-A8; S100 calcium binding protein A8; S100 calcium-binding protein A8 (calgranulin A); S100 calcium-binding protein A8; Urinary stone protein band A
全称	S100 Calcium Binding Protein A8
反应种属	Mouse
应用	Western Blot(2 µg/mL) Immunocytochemistry(8-25 µg/mL)
目标/特异性	Detects mouse S100A8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 40% cross-reactivity with recombinant human (rh) S100A8 is observed and no cross-reactivity with recombinant mouse (rm) S100A4, rmS100A9, rmS100A10, rmS100A11, rhS100B, or rhS100P is observed.
使用方法	Western Blot: 2 µg/mL Immunocytochemistry: 8-25 µg/mL
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6279 (Human); 20201 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Visualized macrophage dynamics and significance of S100A8 in obese fat. Authors: Sekimoto R, Fukuda S, Maeda N, Tsushima Y, Matsuda K, Mori T, Nakatsuji H, Nishizawa H, Kishida K, Kikuta J, Maijima Y, Funahashi T, Ishii M, Shimomura I Proc Natl Acad Sci U S A, 2015;112(16):E2058-66. Species: Mouse Sample Type: Whole Tissue Application: in vitro The calcium-binding protein complex S100A8/A9 has a crucial role in controlling macrophage-mediated renal repair following ischemia/reperfusion. Authors: Dessing, Mark C, Tammaro, Alessand, Pulskens, Wilco P, Teske, Gwendoli, Butter, Loes M, Claessen, Nike, van Eijk, Marco, van der Poll, Tom, Vogl, Thomas, Roth, Johannes, Florquin, Sandrine, Leemans, Jaklien Kidney Int, 2015;87(1):85-94. Species: Mouse Sample Type: Whole Tissue Application: IHC The Acute Neutrophil Response Mediated by S100 Alarmins during Vaginal Candida Infections Is Independent of the Th17-Pathway. Authors: Yano, Junko, Kolls, Jay K, Happel, Kyle I, Wormley, Floyd, Wozniak, Karen L, Fidel, Paul L J PLoS ONE, 2012;7(9):e46311. Species: Mouse Sample Type: Vaginal Wash Application: ELISA Development S100A8/A9 is not involved in host defense against murine urinary tract infection. Authors: Dessing MC, Butter LM, Teske GJ PLoS ONE, 2010;5(10):e13394. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant mouse S100A8 Met1-Glu89 (predicted) Accession # P27005
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Mouse S100A8 (also CP-10, calgranulin A and MRP8) is a 10 kDa member of the S100 family of calcium-binding proteins. The S100A8 protein is 89 amino acids (aa) in length and contains short, sequential modules. There is an N-terminal alpha -helix, followed by a calcium-binding EF-hand segment, and a C-terminal alpha -helix. S100A8 will noncovalently heterodimerize with S100A9. In the presence of calcium, this heterodimer will form a heterotetramer. The dimeric complex is both intracellular and extracellular. It binds heparan sulfate and is chemotactic for PMNs and macrophages. Mouse S100A8 shares 80% and 57% aa sequence identity with rat and human S100A8, respectively.
运输条件	Blue Ice
存放说明	4℃
参考文献	Acute Myeloid Leukemia Calcium-binding Proteins and Related Molecules Granulocyte Markers ICC / IHC Images: S100A8 Inflammatory Mediators Monocyte Markers Myeloid-derived Suppressor Cells (MDSC)

参考图片

S100A8 in NIH-3T3 Mouse Cell Line. S100A8 was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using 10 µg/mL Rat Anti-Mouse S100A8 Monoclonal Antibody (Catalog # MAB3059) for 3 hours at room temperature. Cells were stained (red) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of S100A8 Rat by Western Blot. Western blot shows lysates of mouse leukocyte. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse S100A8 Monoclonal Antibody (Catalog # MAB3059) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for S100A8 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.