Oxidized PTP Active Site MAb (Clone 335636) (25 UG)【科瑞奥博】

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Oxidized PTP Active Site MAb (Clone 335636) (25 UG)

货号： MAB2844-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB2844-SP
别名	Oxidized PTP Active Site
全称	Oxidized Protein Tyrosine Phosphatase Active Site
应用	Western Blot(1 µg/mL)
目标/特异性	Detects Oxidized PTP Active Site. Will react with any oxidized phosphatase containing the consensus sequence. Detects oxidized but not unoxidized DEP-1, PTP1B, TC-PTP, and SHP-2. Other phosphatases have not been tested.
使用方法	Western Blot: 1 µg/mL
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Dual oxidase 1 induced by Th2 cytokines promotes STAT6 phosphorylation via oxidative inactivation of protein tyrosine phosphatase 1B in human epidermal keratinocytes. Authors: Hirakawa S, Saito R, Ohara H J. Immunol., 2011;186(8):4762-70. Species: Human Sample Type: Cell Lysate Application: WB Cysteine S-nitrosylation protects protein-tyrosine phosphatase 1B against oxidation-induced permanent inactivation. Authors: Chen YY, Chu HM, Pan KT, Teng CH, Wang DL, Wang AH, Khoo KH, Meng TC J. Biol. Chem., 2008;283(50):35265-72. Species: Human Sample Type: Cell Lysates Application: WB Redox regulation of interleukin-4 signaling. Authors: Sharma P, Chakraborty R, Wang L, Min B, Tremblay ML, Kawahara T, Lambeth JD, Haque SJ Immunity, 2008;29(4):551-64. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	KLH-coupled perfomic acid oxidized synthetic peptide VHCSAG
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Oxidation of the essential cysteine in the active site of protein tyrosine phosphatases (PTPs) by exogenous or intracellular oxidants such as hydrogen peroxide is believed to play an important role in regulating the activity of these enzymes. The initial oxidation to a sulfenic acid (-SOH) is reversible, but the oxidation often progresses to an irreversible sulfonic acid (-SO₃H) that can be detected by modification-specific antibodies. The active sites of PTPs contain the consensus amino acid sequence (V/I)HCSXG, a sequence essential for catalytic activity.
运输条件	Blue Ice
存放说明	4℃
参考文献	Phosphatases and Regulators Phosphatases in the Akt Pathway

参考图片

Detection of Oxidized PTP by Western Blot. Western blot shows oxidized PTP immunoprecipitated from lysates of HepG2 human hepatocellular carcinoma cell line using Rat Anti-SHP-2 antibody. PVDF membrane was probed with 1 µg/mL Mouse Anti-Oxidized PTP Active Site Monoclonal Antibody (Catalog # MAB2844) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for Oxidized SHP-2 was detected at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.