| 货号 | MAB3196-SP |
| 别名 | DKFZp686F03189; EC 2.7.11; EC 2.7.11.24; ERK-3; ERK3p97-MAPK; Extracellular signal-regulated kinase 3; extracellular signal-regulated kinase, p97; HsT17250; MAP kinase 6; MAP kinase isoform p97; MAPK 6; MAPK6; mitogen-activated protein kinase 6; p97MAPK; PRKM6; protein kinase, mitogen-activated 5; protein kinase, mitogen-activated 6 | 全称 | Extracellular Signal-regulated Kinase 3 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(8-25 µg/mL) |
| 目标/特异性 | Detects human ERK3 in direct ELISA and Western Blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 8-25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5597 (Human); 50772 (Mouse); 58840 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human ERK3 Met344-Asn721 Accession # Q16659 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Extracellular signal-regulated kinase 3 (ERK3) is a serine/threonine protein kinase also known as mitogen-activated protein kinase 6 (MAPK6) and p97MAPK. Unlike ERK1 and ERK2, ERK3 lacks the phosphoacceptor sequence T-E-Y in its activation loop. This kinase also possesses a unique C-terminal extension that is missing in traditional MAPKs. Highly unstable, ERK3 is rapidly degraded following N-terminal ubiquitination. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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ERK3 in Human Breast Cancer Tissue. ERK3 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 8 µg/mL Mouse Anti-Human ERK3 Monoclonal Antibody (Catalog # MAB3196) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. | |
Detection of Human ERK3 by Western Blot. Western blot shows lysates of U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human ERK3 Monoclonal Antibody (Catalog # MAB3196) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ERK3 at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
