| 货号 | MAB3154-SP |
| 别名 | 100 kDa NT receptor; Glycoprotein 95; Gp95; Gp95LDLCQ6; Neurotensin receptor 3; NT3NTR3; Ntr3; SORT1; sortilin 1; sortilin |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(0.25-1 µg/mL) Simple Western(10 µg/mL) |
| 目标/特异性 | Detects human, mouse, and rat Sortilin in Western blots. |
| 使用方法 | Western Blot: 0.25-1 µg/mL Simple Western: 10 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6272 (Human); 20661 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Sortilin Ser78-Asn755 Accession # Q99523 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Sortilin, also known as neurotensin receptor 3, is a 100 kDatransmembrane receptor that is localized both in the endosomalcompartment and on plasma membranes. It contains anextracellular/lumenal Vps10p (vacuolar protein-sorting 10 protein)domain and is one of five known members of the mammalian Vps10p-Dreceptor family. A Sortilin propeptide, cleaved by a furin, blockspremature ligand binding to uncleaved Sortilin and functions as achaperone to facilitate trafficking in the Golgi compartments. Sortilinbinds multiple ligands and has diverse functions. Mature human Sortilinextracellular domain shares 92% and 91% amino acid sequence identitywith that of mouse and rat Sortilin, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Sortilin by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue and human brain (hippocampus) tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human/Mouse/Rat Sortilin Monoclonal Antibody (Catalog # MAB3154) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Sortilin at approximately 95-105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of Human Sortilin by Simple WesternTM. Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for Sortilin at approximately 87 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat Sortilin Monoclonal Antibody (Catalog # MAB3154). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. | |
Detection of Mouse and Rat Sortilin by Western Blot. Western blot shows lysates of mouse brain tissue and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Sortilin Monoclonal Antibody (Catalog # MAB3154) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Sortilin at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
