| 货号 | MAB30861-SP |
| 别名 | FLJ14594; LERN1; LERN1UNQ201; leucine rich repeat and Ig domain containing 1; leucine rich repeat neuronal 6A; Leucine-rich repeat and immunoglobilin domain-containing protein 1; Leucine-rich repeat neuronal protein 1; Leucine-rich repeat neuronal protein 6A; LRRN6A; LRRN6Aleucine-rich repeat neuronal 6A; MGC17422 | 全称 | Leucine-rich Repeat And Ig Domain Containing Nogo Receptor-Interacting Protein 1 |
| 反应种属 | Human |
| 应用 | Flow Cytometry(2.5 µg/106cells) Immunocytochemistry(8-25 µg/mL) |
| 目标/特异性 | Detects human LINGO-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human (rh) LINGO-2 is observed and no cross-reactivity with rhLINGO-3 is observed. |
| 使用方法 | Flow Cytometry: 2.5 µg/106cells Immunocytochemistry: 8-25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 84894 (Human) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. LINGO-1 protein interacts with the p75 neurotrophin receptor in intracellular membrane compartments. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human LINGO-1 Thr40-Thr556 Accession # NP_116197 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | LINGO-1 (LRRN6A) is a 614 amino acid (aa) transmembrane protein of the leucine-rich repeat (LRR) family, ribonuclease inhibitor subfamily. The four known LINGO proteins contain LRR and IgCAM domains in the extracellular portion and share 44‑61% aa sequence identity. LINGO-1 is restricted to the nervous system and is concentrated in the brain as a component of the NgR1/p75 and NgR1/Taj (TROY) signaling complexes. LINGO-1 negatively regulates neurite outgrowth and myelination. LINGO-1 is highly conserved, showing 99% aa sequence identity between human, mouse and rat. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of LINGO‑1 in CHP‑100 Human Cell Line by Flow Cytometry. CHP‑100 human neuroblastoma cell line was stained with Mouse Anti-Human LINGO‑1 Monoclonal Antibody (Catalog # MAB30861, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab)2 Secondary Antibody (Catalog # F0101B). |
