| 货号 | MAB214-SP |
| 别名 | C17orf33; chromosome 17 open reading frame 33; colony stimulating factor 3 (granulocyte); CSF3; CSF3OS; Filgrastim; G-CSF; GCSFlenograstim; granulocyte colony-stimulating factor; Lenograstim; MGC45931; pluripoietin | 全称 | Granulocyte Colony Stimulating Factor |
| 反应种属 | Human |
| 应用 | ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( ) |
| 目标/特异性 | Captures human G-CSF in sandwich ELISAs. In this format, no cross-reactivity or interference was observed with recombinant human (rh) GM-CSF, rmGM-CSF, rhM-CSF, or rmM-CSF. This antibody cross-reacts with recombinant mouse G-CSF at concentrations greater than 50 ng/mL. |
| 使用方法 | ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize G‑CSF-induced proliferation in the NFS‑60 mouse myeloid cell line. Shirafuji, N.et al. (1989) Exp. Hematol. 17:116. The Neutralization Dose (ND50) is typically 0.01-0.03 µg/mL in the presence of 0.125 ng/mL Recombinant Human G‑CSF. |
| 来源 | Monoclonal Mouse IgG1 Clone # 3316 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1440 (Human); 12985 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Proteinase-activated receptor2 agonists upregulate granulocyte colony-stimulating factor, IL-8, and VCAM-1 expression in human bronchial fibroblasts. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human G-CSF Ala30-Pro204 Accession # NP_757373 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma. Other cell types including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells can also secrete G-CSF after LPS, IL-1, or TNF-alpha activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively. In humans, two distinct cDNA clones for G-CSF, encoding 207 and 204 amino acid precursor proteins, have been isolated. Both proteins have a 30 amino acid signal peptide and have identical amino acid sequences except for a three amino acid insertion (deletion) at the 35th amino acid residue from the N-terminus of the mature protein. Human G-CSF is 73% identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity. In vitro, G-CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage. It also has blast cell growth factor activity and can synergize with IL-3 to shorten the Go period of early hematopoietic progenitors. Consistent with its in vitro functions, G-CSF has been found to play important roles in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Cell Proliferation Induced by G‑CSF and Neutralization by Human G‑CSF Antibody. Recombinant Human G‑CSF (Catalog # 214-CS) stimulates proliferation in the NFS‑60 mouse myeloid cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human G‑CSF (0.125 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human G‑CSF Monoclonal Antibody (Catalog # MAB214). The ND50 is typically 0.01-0.03 µg/mL. |
