货号 | MAB3110-SP |
别名 | Dermatan sulfate proteoglycan 3dermatan sulphate proteoglycan 3; DSPG3proteoglycan-lb; Epiphycan; EPYC; PGLB; PG-Lb; Proteoglycan-Lb; SLRR3B; SLRR3Bepiphycan proteoglycan; Small chondroitin/dermatan sulfate proteoglycan | 全称 | Dermatan Sulfate Proteoglycan 3 |
反应种属 | Human |
应用 | Western Blot(1 µg/mL) Immunocytochemistry(8-25 µg/mL) |
目标/特异性 | Detects human DSPG3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse DSPG3 is observed. |
使用方法 | Western Blot: 1 µg/mL Immunocytochemistry: 8-25 µg/mL |
来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 1833 (Human); 13516 (Mouse) |
纯化方式 | Protein A or G purified from hybridoma culture supernatant |
免疫原 | Mouse myeloma cell line NS0-derived recombinant human DSPG3 Ala20-Val322 Accession # Q99645 |
生物活性 | Human |
标记 | Unconjugated |
溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
背景 | DSPG3 is a secreted protein in the SLRP (small leucine-rich proteoglycan) family. The mature protein contains multiple sites for O-linked glycanation and seven leucine-rich repeats, flanked by N- and C-terminal cysteine-rich regions. DSPG3 is principally a component of cartilage. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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DSPG3 in Human HUVEC. DSPG3 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Mouse Anti-Human DSPG3 Monoclonal Antibody (Catalog # MAB3110) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |