| 货号 | MAB2964-SP |
| 别名 | FABPL; fatty acid binding protein 1, liver; fatty acid-binding protein, liver; L-FABP; L-FABPFatty acid-binding protein 1; Liver-type fatty acid-binding protein | 全称 | Fatty Acid-Binding Protein 1 |
| 反应种属 | Human |
| 应用 | Western Blot(0.25 µg/mL) Immunohistochemistry(8-25 µg/mL) |
| 目标/特异性 | Detects human FABP1/L‑FABP in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human FABP2, -3, -4, -5, -6, -7, or recombinant mouse FABP9 is observed. |
| 使用方法 | Western Blot: 0.25 µg/mL Immunohistochemistry: 8-25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2168 (Human); 14080 (Mouse); 24360 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human FABP1/L‑FABP Met1-Ile127 Accession # AAA52418 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | FABP1, also known as liver FABP (L-FABP, Z-protein and squalene-andsterol-carrier protein [SCP]) is a member of the intracellular FABPfamily. It is highly expressed in the liver, intestine, kidney andlung. FABP1 binds free fatty acids and their co-enzyme A derivativesand may be involved in intracellular lipid transport. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human FABP1/L‑FABP by Western Blot. Western blot shows lysates of human liver tissue, human colon tissue, and human kidney tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human FABP1/L‑FABP Monoclonal Antibody (Catalog # MAB2964) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FABP1/L‑FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
FABP1/L‑FABP in Human Kidney. FABP1/L‑FABP was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human FABP1/L‑FABP Monoclonal Antibody (Catalog # MAB2964) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
