| 货号 | MAB301-SP |
| 别名 | CD123 antigen; CD123; hIL3Ra; hIL-3Ra; IL-3 receptor subunit alpha; IL-3R subunit alpha; IL3R; IL3RA; IL-3RA; IL-3R-alpha; IL3RAY; IL3RX; IL3RY; interleukin 3 receptor, alpha (low affinity); interleukin-3 receptor subunit alpha; MGC34174 | 全称 | Interleukin 3 Receptor alpha |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Flow Cytometry(2.5 µg/106cells) Immunohistochemistry(8-25 µg/mL) Immunocytochemistry(8-25 µg/mL) |
| 目标/特异性 | Detects human IL‑3 R alpha /CD123 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Flow Cytometry: 2.5 µg/106cells Immunohistochemistry: 8-25 µg/mL Immunocytochemistry: 8-25 µg/mL Neutralization: Measured by its ability to neutralize IL‑3-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.6-1.2 µg/mL in the presence of 0.5 ng/mL Recombinant Human IL‑3. |
| 来源 | Monoclonal Mouse IgG1 Clone # 32703 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3563 (Human); 16188 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Human T-cell leukemia virus type 2 induces survival and proliferation of CD34(+) TF-1 cells through activation of STAT1 and STAT5 by secretion of interferon-gamma and granulocyte macrophage-colony-stimulating factor. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑3 R alpha /CD123 Lys20-Arg305, predicted Accession # P26951 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | IL-3 is a pleiotropic cytokine that can stimulate proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors (1, 2). IL-3 exerts its activity through binding to a specific cell surface receptor known as IL-3 R. IL-3 R is a heterodimeric structure composed of a 70 kDa IL-3 R alpha subunit (CD123) and a 120-140 kDa IL-3 R beta subunit (CD131) (3, 4). IL-3 R alpha binds IL-3 with relatively low affinity. In the presence of IL-3 R beta, however, IL-3 R alpha has a much higher affinity for IL-3. It is not clear how signal transduction occurs following IL-3 binding. The IL-3 R alpha chain has a very short intracellular domain while the IL‑3 R beta chain has a very large cytoplasmic domain. The IL‑3 R beta chain is also shared by the receptors for IL-5 and GM-CSF. Cells known to express IL-3 receptors include hematopoietic progenitors, epithelial cells, double negative T cells, mast cells, basophils and blood monocytes (5). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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IL‑3 R alpha /CD123 in Human Peripheral Blood Lymphocytes. IL‑3 R alpha /CD123 was detected in immersion fixed human peripheral blood lymphocytes using 2 µg/mL Mouse Anti-Human IL‑3 R alpha /CD123 Monoclonal Antibody (Catalog # MAB301) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells. | |
IL‑3 R alpha /CD123 in Human Tonsil. IL‑3 R alpha /CD123 was detected in immersion fixed paraffin-embedded sections of human tonsil using 15 µg/mL Mouse Anti-Human IL‑3 R alpha /CD123 Monoclonal Antibody (Catalog # MAB301) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. | |
Cell Proliferation Induced by IL‑3 and Neutralization by Human IL‑3 R alpha /CD123 Antibody. Recombinant Human IL‑3 (Catalog # 203-IL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑3 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑3 R alpha /CD123 Monoclonal Antibody (Catalog # MAB301). The ND50 is typically 0.6‑1.2 µg/mL. |
