| 货号 | MAB2798-SP |
| 别名 | Sleeping Beauty Transposase |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(8-25 µg/mL) |
| 目标/特异性 | Detects Sleeping Beauty (SB) Transposase in Western blots. In Immunohistochemistry tests, this antibody stains SB transposase transgenic mouse tissue sections but not wild type mouse tissue sections. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 8-25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Transposon mutagenesis identifies genes and cellular processes driving epithelial-mesenchymal transition in hepatocellular carcinoma | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant Sleeping Beauty Transposase |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | SB transposase is the catalytic component of a DNA transposition system that catalyzes the excision of the transposon from its original location and promotes its reintegration elsewhere in the genome. An active transposase that acts on transposon sequences found in vertebrate genomes was reconstructed from consensus sequences of several mutated and inactive Tc1/mariner family transposase genes in salmonid fish. The entire 340 amino aa transposase sequence contains a paired-like domain with a leucine zipper for DNA binding, a nuclear localization sequence (NLS), a glycine-rich box and a DD(34)E box. The recognition domain for transposon DNA is contained within the first 123 aa of the transposase (1). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Sleeping Beauty Transposase in Mouse Liver. Sleeping Beauty Transposase (SBT) was detected in perfusion fixed paraffin-embedded sections of liver from SBT transgenic mice using 5 µg/mL Mouse Anti-Sleeping Beauty Transposase Monoclonal Antibody (Catalog # MAB2798) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained (brown). Inset panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. | |
Sleeping Beauty Transposase in Mouse Intestine. Sleeping Beauty Transposase (SBT) was detected in perfusion fixed paraffin-embedded sections of intestine from SBT transgenic mice using 5 µg/mL Mouse Anti-Sleeping Beauty Transposase Monoclonal Antibody (Catalog # MAB2798) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained (brown). Inset panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
