| 货号 | MAB2184-SP |
| 别名 | CA2; CAII; CA-IIEC 4.2.1.1; Car2; Carbonate dehydratase II; carbonic anhydrase 2; carbonic anhydrase B; Carbonic anhydrase C; carbonic anhydrase IICAC; carbonic dehydratase |
| 反应种属 | Human |
| 应用 | Western Blot(2 µg/mL) |
| 目标/特异性 | Detects human Carbonic Anhydrase II/CA2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) CA-1, -3, -4, -5A, -5B, -6, -7, -8, or -9 is observed. |
| 使用方法 | Western Blot: 2 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 760 (Human) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human Carbonic Anhydrase II/CA2 Ser2-Lys260 Accession # P00918 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in the CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from use of CAs as markers for tumor and hypoxia in the clinic, as nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA2 is a cytosolic enzyme with the highest activity among all known CAs. Mutations in the CA2 gene result in the CA II deficiency syndrome, an autosomal recessive disorder that produces osteopetrosis, renal tubular acidosis and cerebral calcification (2). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human and Mouse Carbonic Anhydrase II/CA2 by Western Blot. Western blot shows lysates of human kidney tissue, Caki‑2 human clear cell carcinoma epithelial cell line, and RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse Carbonic Anhydrase II/ CA2 Monoclonal Antibody (Catalog # MAB2184) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Carbonic Anhydrase II/CA2 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
