Human Coagulation Factor VII MAb (Clone 321605) (25 UG)【科瑞奥博】

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Human Coagulation Factor VII MAb (Clone 321605) (25 UG)

货号： MAB23381-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB23381-SP
别名	coagulation factor VII (serum prothrombin conversion accelerator); coagulation factor VII; EC 3.4.21; EC 3.4.21.21; Eptacog alfa; F7; FVII coagulation protein; proconvertin; Serum prothrombin conversion accelerator; SPCA
全称	Coagulation Factor VII (Serum Prothrombin Conversion Accelerator)
反应种属	Human
应用	Flow Cytometry(0.25 µg/10⁶cells)
目标/特异性	Detects human Coagulation Factor VII in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Kallikrein‑1, -2, -3, -4, -5, -7, -8, -9, -10, -11, -12, -13, -14, -15, -B1, rhHGFA, rhFactor X, rhFactor XI, rhThrombin, rhuPA, or rhPROC is observed.
使用方法	Flow Cytometry: 0.25 µg/10⁶cells
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2155 (Human); 14068 (Mouse)
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse myeloma cell line NS0-derived recombinant human Coagulation Factor VII Ala39-Pro444 Accession # NP_062562
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Coagulation Factors VII and VIIa refer to the pro and active forms of the same protease, respectively (1). Factor VII is synthesized in the liver and circulates in the plasma where it binds to tissue factor (TF), an integral membrane protein found in a variety of cell types. Upon binding of TF, Factor VII is rapidly converted into VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and has also important coagulation-independent functions such as angiognesis (2). The cleavage and activation of Coagulation Factors VII, IX and X by VIIa:TF is phospholipid-dependent whereas the cleavage of small peptide substrates is not (1). The predominant splicing variant of Factor VII in normal liver corresponds to the 444 amino acid precursor (3, 4). After a signal peptide (residues 1-38), the mature chain can be further processed into the light chain (residues 39-190) and the heavy chain (residues 191-444). The purified rhFactor VII corresponds to the mature chain, which can be processed and activated by treatment with thermolysin and binding with rhTissue Factor (R&D Systems, Catalog # 2339-PA) under the conditions described above.
运输条件	Blue Ice
存放说明	4℃
参考文献	Morrissey, J.H. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. p. 1659. Versteeg, H.H. et al. (2003) Carcinogenesis 24:1009. Hagen, F.S. et al. (1986) Proc. Natl. Acad. Sci. USA 83:2412. O’Hara, P.J. et al. (1987) Proc. Natl. Acad. Sci. USA 84:5158.

参考图片

Detection of Coagulation Factor VII in HASMC Human Cells by Flow Cytometry. HASMC human aortic smooth muscle cells were stained with Mouse Anti-Human Coagulation Factor VII Monoclonal Antibody (Catalog # MAB23381, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab)₂ Secondary Antibody (Catalog # F0102B).