| 货号 | MAB2927-SP |
| 别名 | CALR3; calreticulin 2; calreticulin 3; Calreticulin-2; calreticulin-3; cancer/testis antigen 93; Crt2; CRT2calreticulin-2; CT93; FLJ25355; MGC26577 |
| 反应种属 | Human |
| 应用 | Intracellular Staining by Flow Cytometry(2.5 µg/106cells) |
| 目标/特异性 | Detects human Calreticulin-2 in direct ELISAs. In direct ELISAs, no cross-reactivitywith recombinant human Calreticulin is observed. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 2.5 µg/106cells |
| 来源 | Sterile PBS to a final concentration of 0.5 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 125972 (Human); 73316 (Mouse); 364529 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human Calreticulin-2 Thr20-Leu384 Accession # Q96L12 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.5 mg/mL. |
| 背景 | Calreticulin-2 is a soluble lectin-like molecule that participates in the protein folding and quality control cycle in the endoplasmic reticulum (ER) lumen. Mature human Calreticulin-2 has a 178 amino acid N-terminal globular domain, a 97 aa central P-domain, and a 90 aa C-terminal domain. The globular domain shows lectin activity, the P-domain binds one Ca++ ion with high affinity, and the C-terminus binds Ca++ and contains an RNEL ER retention motif. The amino acid sequence of Calreticulin‑2 is 84%, 83% and 87% identical to that of mouse, bovine and canine Calreticulin‑2, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Calreticulin-2 in MCF‑7 Human Cell Line by Flow Cytometry. MCF‑7 human breast cancer cell line was stained with Mouse Anti-Human Calreticulin‑2 Monoclonal Antibody (Catalog # MAB2927, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin. |
