Human GM-CSF MAb (Clone 3209) (25 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human GM-CSF MAb (Clone 3209) (25 UG)

货号： MAB215-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB215-SP
别名	colony stimulating factor 2 (granulocyte-macrophage); Colony-stimulating factor; CSF; CSF2; GM-CSF; GMCSFgranulocyte-macrophage colony-stimulating factor; granulocyte-macrophage colony stimulating factor; MGC131935; MGC138897; molgramostin; sargramostim
全称	Granulocyte Macrophage Growth Factor
反应种属	Human
应用	Western Blot(1 µg/mL) Immunocytochemistry(8-25 µg/mL)
目标/特异性	Detects human GM-CSF in Western blots.
使用方法	Western Blot: 1 µg/mL Immunocytochemistry: 8-25 µg/mL Neutralization: Measured by its ability to neutralize GM‑CSF-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 0.3-0.5 µg/mL in the presence of 0.5 ng/mL Recombinant Human GM‑CSF.
来源	Monoclonal Mouse IgG₁ Clone # 3209
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	1437 (Human); 12981 (Mouse); 116630 (Rat); 397208 (Porcine); 403923 (Canine); 493805 (Feline)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Innate lymphoid cells integrate stromal and immunological signals to enhance antibody production by splenic marginal zone B cells. Authors: Magri G, Miyajima M, Bascones S, Mortha A, Puga I, Cassis L, Barra C, Comerma L, Chudnovskiy A, Gentile M, Llige D, Cols M, Serrano S, Arostegui J, Juan M, Yague J, Merad M, Fagarasan S, Cerutti A Nat Immunol, 2014;15(4):354-64. Species: Human Sample Type: Whole Cells Application: Neut The implication of aberrant GM-CSF expression in decidual cells in the pathogenesis of preeclampsia. Authors: Huang SJ, Zenclussen AC, Chen CP, Basar M, Yang H, Arcuri F, Li M, Kocamaz E, Buchwalder L, Rahman M, Kayisli U, Schatz F, Toti P, Lockwood CJ Am. J. Pathol., 2010;177(5):2472-82. Species: Human Sample Type: Whole Cells Application: Neut Regulation of human skin pigmentation in situ by repetitive UV exposure: molecular characterization of responses to UVA and/or UVB. Authors: Choi W, Miyamura Y, Wolber R, Smuda C, Reinhold W, Liu H, Kolbe L, Hearing VJ J. Invest. Dermatol., 2010;130(6):1685-96. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Human epithelial cells establish direct antifungal defense through TLR4-mediated signaling. Authors: Weindl G, Naglik JR, Kaesler S, Biedermann T, Hube B, Korting HC, Schaller M J. Clin. Invest., 2007;117(12):3664-3672. Species: Human Sample Type: Whole Cells Application: Neut Microvascular endothelial cells increase proliferation and inhibit apoptosis of native human acute myelogenous leukemia blasts. Authors: Hatfield K, Ryningen A, Corbascio M, Bruserud O Int. J. Cancer, 2006;119(10):2313-21. Species: Human Sample Type: Whole Cells Application: Neut Differential survival of leukocyte subsets mediated by synovial, bone marrow, and skin fibroblasts: site-specific versus activation-dependent survival of T cells and neutrophils. Authors: Filer A, Parsonage G, Smith E, Osborne C, Thomas AM, Curnow SJ, Rainger GE, Raza K, Nash GB, Lord J, Salmon M, Buckley CD Arthritis Rheum., 2006;54(7):2096-108. Species: Human Sample Type: Cell Culture Supernates Application: Immunodepletion Regulation of the interleukin-5 receptor alpha-subunit on peripheral blood eosinophils from healthy subjects. Authors: Hellman C, Hallden G, Hylander B, Lundahl J Clin. Exp. Immunol., 2003;131(1):75-81. Species: Human Sample Type: Whole Cells Application: Neut Thymus and activation-regulated chemokine (TARC/CCL17) produced by mouse epidermal Langerhans cells is upregulated by TNF-alpha and IL-4 and downregulated by IFN-gamma. Authors: Xiao T, Fujita H, Saeki H, Mitsui H, Sugaya M, Tada Y, Kakinuma T, Torii H, Nakamura K, Asahina A, Tamaki K Cytokine, 2003;23(4):126-32. Species: Human Sample Type: Whole Tissue Application: IHC Frozen Human T-cell leukemia virus type 2 induces survival and proliferation of CD34(+) TF-1 cells through activation of STAT1 and STAT5 by secretion of interferon-gamma and granulocyte macrophage-colony-stimulating factor. Authors: Bovolenta C, Pilotti E, Mauri M, Turci M, Ciancianaini P, Fisicaro P, Bertazzoni U, Poli G, Casoli C Blood, 2002;99(1):224-31. Species: Human Sample Type: Whole Cells Application: Neut
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human GM-CSF Ala18-Glu144 Accession # P04141
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) is a growth factor produced by a variety of lymphoid cells. It binds to receptor heterodimers consisting of a GM-CSF-specific alpha chain and the common beta chain that is shared by the high-affinity receptors for IL-3 and IL-5.
运输条件	Blue Ice
存放说明	4℃
参考文献	Common beta Chain Receptor Family Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis Dendritic Cell Development Gametes and Fertilization gamma delta T Cells ICC / IHC Images: GM-CSF Inflammatory Mediators Isolation, Culture, and Identification of Natural Killer Cells Macrophage Activation Markers Myeloid-derived Suppressor Cells (MDSC) Natural Killer T (NKT) Cells T Cell Cytokine Signaling

参考图片

Cell Proliferation Induced by GM‑CSF and Neutralization by Human GM‑CSF Antibody. Recombinant Human GM‑CSF (Catalog # 215-GM) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human GM‑CSF (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Human GM‑CSF Monoclonal Antibody (Catalog # MAB215). The ND₅₀ is typically 0.3-0.5 µg/mL.

GMCSF in Human PBMCs. Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) was detected in human peripheral blood mononuclear cells (PBMCs) using Human GM-CSF Monoclonal Antibody (Catalog # MAB215) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.