| 货号 | MAB24601-SP |
| 别名 | coagulation factor XI; EC 3.4.21; EC 3.4.21.27; FXIPlasma thromboplastin antecedent; MGC141891; PTA |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunoprecipitation(25 µg/mL) |
| 目标/特异性 | Detects human Coagulation Factor XI Light Chain in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) UPA-1, rhThrombin, rhKLK1, rhKLK3, rhKLK4, rhKLK5, rhKLK6, rhKLK8, rhKLK11, rhHGFA, rhFactor VII, or rhFactor X is observed. This antibody recognizes mature chain (aa 19‑625), light chain (aa 388‑625) and the active enzyme, which contains disulfide bond-linked light and heavy chains. |
| 使用方法 | Western Blot: 1 µg/mL Immunoprecipitation: 25 µg/mL Neutralization: Measured by its ability to neutralize Recombinant Human Coagulation Factor XI (2 µg/mL, Catalog # 2460-SE) cleavage of the fluorogenic peptide substrate Boc-IEGR-Amc(100 µM). The Neutralization Dose (ND50) is typically 2.5 µg/mL. |
| 来源 | Monoclonal Mouse IgG2B Clone # 318104 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2160 (Human); 109821 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Coagulation Factor XI Glu19-Val625 Accession # P03951 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Coagulation factors XI and XIa refer to the pro and active forms of the same protease, respectively (1). Factor XI is synthesized in the liver and circulates in the plasma as a disulfide bond-linked dimer complexed with high molecular weight kininogen. Factor XI is converted into XIa via either the contact phase of blood coagulation or thrombin-mediated activation on the platelet surface. The resulting XIa converts factor IX into IXa, which subsequently activates factor X into Xa. Factor Xa in turn activates factor II/thrombin to complete the coagulation cascade. Patients with factor XI deficiency are prone to excessive bleeding after hemostatic challenge. There are two alternative splicing forms. Isoform 1 corresponds to the circulating plasma factor XI and isoform 2 is produced by platelets and megakaryocytes but absent from other blood cells (2). The 625 amino acid (aa) precursor of isoform 1 consists of a signal peptide (aa 1-18) and the mature chain (aa 19-625). The mature chain (XI) can be further processed into the heavy chain (aa 19-387) and the light chain (aa 388-625) (XIa). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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