Carboxymethyl Lysine MAb (Clone 318003) (25 UG)【科瑞奥博】

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Carboxymethyl Lysine MAb (Clone 318003) (25 UG)

货号： MAB3247-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB3247-SP
别名	CML
应用	Western Blot(1 µg/mL)
目标/特异性	Detects Carboxymethyl Lysine adducts on proteins in Western blots. In Western blots, no cross-reactivity with carboxyethyl lysine adducts is observed.
使用方法	Western Blot: 1 µg/mL
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. An advanced glycation end product (AGE)-receptor for AGEs (RAGE) axis restores adipogenic potential of senescent preadipocytes through modulation of p53 protein function. Authors: Chen, Chih-Yu, Abell, Allison, Moon, Yang Soo, Kim, Kee-Hong J Biol Chem, 2012;287(53):44498-507. Species: Mouse Sample Type: Cell Lysate Application: WB Advanced glycation end products accumulate in vascular smooth muscle and modify vascular but not ventricular properties in elderly hypertensive canines. Authors: Shapiro BP, Owan TE, Mohammed SF, Meyer DM, Mills LD, Schalkwijk CG, Redfield MM Circulation, 2008;118(10):1002-10. Species: Canine Sample Type: Tissue Homogenates Application: WB
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Carboxymethyl Lysine-modified Keyhole Limpet Hemocyanin (KLH)
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	N epsilon -carboxymethyl lysine (CML) is formed by the non-enzymatic Schiff-base reaction of glucose with proteins, followed by an Amadori rearrangement and oxidation that leaves only a carboxymethyl group attached to the lysine. The levels of CML adducts accumulate over time and have been used as an indicator of both serum glucose levels and oxidative protein damage. Elevated serum CML-modified proteins have been associated with diabetes and may contribute to diabetic retinopathy, nephropathy and angiopathy.
运输条件	Blue Ice
存放说明	4℃
参考文献	Oxidative Stress Pro-oxidant Molecules ROS Sensors, Adducts, and Inhibitor Molecules

参考图片

Detection of CML-adducted Protein by Western Blot. Western blot shows 100-fold diluted samples of sera from control, Type 1 diabetic, and Type 2 diabetic volunteers. PVDF membrane was probed with 1 µg/mL Carboxymethyl Lysine Monoclonal Antibody (Catalog # MAB3247) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Greater band intensity for the IgG heavy chain (IgG hc) suggests increased glycation in diabetic sera. For additional reference, carboxymethyl lysine-adducted (CML) Leptin and carboxyethyl lysine-adducted (CEL) Leptin were included. A specific band for CML-adducted Leptin was detected (as indicated). This experiment was conducted under reducing conditions using Immunoblot Buffer Group 1.