| 货号 | MAB3294-SP |
| 别名 | CDLS2; DXS423ESMC1 structural maintenance of chromosomes 1-like 1 (yeast); KIAA0178DKFZp686L19178; SB1.8; SB1.8MGC138332; segregation of mitotic chromosomes 1; SMC protein 1A; SMC1 (structural maintenance of chromosomes 1, yeast)-like 1; SMC1 structural maintenance of chromosomes 1-like 1; SMC1A; SMC-1A; SMC-1-alpha; SMC1L1; SMC1SMC1alpha; Smcb; structural maintenance of chromosomes 1A; structural maintenance of chromosomes protein 1A | 全称 | Structural Maintenance of Chromosomes 1 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(0.25 µg/mL) |
| 目标/特异性 | Detects human, mouse and rat SMC1 in Western blots. |
| 使用方法 | Western Blot: 0.25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8243 (Human); 63996 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human SMC1 Met837-Gln1233 Accession # Q14683 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | StructuralMaintenance of Chromosome1 (SMC1) is an ATPase which, with SMC3, forms a heterodimeric complex. These dimers form a V-shaped molecule with two long coiled-coil arms, each having an ATP-binding domain at its distal end. The SMC1/SMC3 heterodimer plays an important role in sister chromatid cohesion during metaphase, associates with the nuclear matrix and centrosome during interphase and localizes to kinetochores, spindles and spindle poles during mitosis. Smc1 is also a component of the DNA damage response and participates in the cellular response to DNA damage through its phosphorylation on Ser 957 and 966. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human/Mouse/Rat SMC1 by Western Blot. Western blot shows lysates of U2OS human osteosarcoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.25 µg/mL of Human/Mouse/Rat SMC1 Monoclonal Antibody (Catalog # MAB3294) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SMC1 at approximately 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
