Human CXCL1/2/3/GRO Pan Specific MAb (Clone 31716) (25 UG)【科瑞奥博】

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Human CXCL1/2/3/GRO Pan Specific MAb (Clone 31716) (25 UG)

货号： MAB276-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB276-SP
别名	CXCL1/2/3/GRO
反应种属	Human
应用	Western Blot(1 µg/mL)
目标/特异性	Detects human CXCL1/2/3 in direct ELISAs and Western blots.
使用方法	Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize CXCL1/2/3/GRO-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND₅₀) is typically 0.5-2.5 µg/mL, 0.15-0.75µg/mL, and 0.1-0.5 µg/mL for Recombinant Human (rh) CXCL1, rhCXCL2, and rhCXCL3, respectively.
来源	Monoclonal Mouse IgG₁ Clone # 31716
产品组分

性能
供应商	R&D Systems
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Podocytes regulate neutrophil recruitment by glomerular endothelial cells via IL-6-mediated crosstalk. Authors: Kuravi S, McGettrick H, Satchell S, Saleem M, Harper L, Williams J, Rainger G, Savage C J Immunol, 2014;193(1):234-43. Species: Human Sample Type: Whole Cells Application: Neut CXCR2-driven ovarian cancer progression involves upregulation of proinflammatory chemokines by potentiating NF-kappaB activation via EGFR-transactivated Akt signaling. Authors: Dong Y, Kabir S, Lee E, Son D PLoS ONE, 2013;8(12):e83789. Species: Human Sample Type: Whole Cells Application: Neut Skin Inflammation Induced by the Synergistic Action of IL-17A, IL-22, Oncostatin M, IL-1alpha, and TNF-alpha Recapitulates Some Features of Psoriasis. Authors: Guilloteau K, Paris I, Pedretti N, Boniface K, Juchaux F, Huguier V, Guillet G, Bernard FX, Lecron JC, Morel F J. Immunol., 2010;184(0):5263-70. Species: Human Sample Type: Whole Cells Application: Neut Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine. Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ Transplantation, 2009;87(12):1814-20. Species: Human Sample Type: Urine Application: Antibody Array Development L5, the most electronegative subfraction of plasma LDL, induces endothelial vascular cell adhesion molecule 1 and CXC chemokines, which mediate mononuclear leukocyte adhesion. Authors: Abe Y, Fornage M, Yang CY, Bui-Thanh NA, Wise V, Chen HH, Rangaraj G, Ballantyne CM Atherosclerosis, 2007;192(1):56-66. Species: Human Sample Type: Whole Cells Application: Neut Laminar shear stress-induced GRO mRNA and protein expression in endothelial cells. Authors: Hagiwara H, Mitsumata M, Yamane T Circulation, 1998;98(23):2584-90. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Protein A or G purified from ascites
免疫原	E. coli-derived recombinant human CXCL2 Ala35-Asn107 Accession # P19875
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	CXCL1, CXCL2 (MIP-2 alpha ), and CXCL3 (MIP-2 beta ) are members of the alpha (C-X-C) subfamily of chemokines and are now designated GRO alpha, GRO beta, and GRO gamma, respectively. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators.
运输条件	Blue Ice
存放说明	4℃
参考文献	Chemokines and Receptors in Allergy and Asthma Chemokines and Receptors in Angiogenesis CXC Chemokines and Receptors

参考图片

Chemotaxis Induced by CXCL2/GRO beta and Neutralization by Human CXCL1/2/3/GRO Antibody. Recombinant Human CXCL2/GRO beta (Catalog # 176-GB) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL2/GRO beta (6 ng/mL) is neutralized (green line) by increasing concentrations of Human CXCL1/2/3/GRO Monoclonal Antibody (Catalog # MAB276). The ND₅₀ is typically 0.15-0.75 µg/mL.