货号 | MAB29281-SP |
别名 | MYD88D; myeloid differentiation primary response gene (88); myeloid differentiation primary response protein MyD88 | 全称 | Myeloid Differentiation Primary Response Gene 88 |
反应种属 | Human |
应用 | Intracellular Staining by Flow Cytometry(0.25 µg/106cells) |
目标/特异性 | Detects human MyD88 in direct ELISAs. In direct ELISAs, no cross-reactivity with mouse MyD88 is observed. |
使用方法 | Intracellular Staining by Flow Cytometry: 0.25 µg/106cells |
来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 4615 (Human); 17874 (Mouse) |
纯化方式 | Protein A or G purified from hybridoma culture supernatant |
免疫原 | E. coli-derived recombinant human MyD88 Met1-Pro296 Accession # Q99836 |
生物活性 | Human |
标记 | Unconjugated |
溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
背景 | Myeloid Differentiation primary response gene 88 (MyD88) contains a cytoplasmic Toll/interleukin 1 receptor (TIR) and functions as an adaptor protein in Toll-like receptor signaling. It is expressed in many adult tissues including heart, kidney and liver tissue and in monocyte, T, B, NK and dendritic cells. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Detection of MyD88 in Raji Human Cell Line by Flow Cytometry. Raji human Burkitts lymphoma cell line was stained with Rat Anti-Human MyD88 Monoclonal Antibody (Catalog # MAB29281, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab)2 Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin. |