| 货号 | MAB2698-SP |
| 别名 | IREL; I-Rel; nuclear factor of kappalight polypeptide gene enhancer in B-cells 3; v-rel reticuloendotheliosis viral oncogene homolog B | 全称 | v-rel Reticuloendotheliosis Viral Oncogene Homolog B |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(8-25 µg/mL) Immunocytochemistry(8-25 µg/mL) |
| 目标/特异性 | Detects human RelB. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 8-25 µg/mL Immunocytochemistry: 8-25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5971 (Human) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Kinome-wide functional genomics screen reveals a novel mechanism of TNFα-induced nuclear accumulation of the HIF-1α transcription factor in cancer cells. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human RelB Gln382-Thr579 Accession # Q01201 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | RelB is a member of the NFkB family and can act as either an activator or repressor of transcription by forming heterodimers with the p50 and p52 NFkB family members. Although RelB knock out mice are viable, there are complex abnormalities in their inflammatory response, hematopoietic lineage and formation of secondary lymphoid structures. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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RelB in Human Lymphoma. RelB was detected in immersion fixed paraffin-embedded sections of human lymphoma using 8 µg/mL Mouse Anti-Human RelB Monoclonal Antibody (Catalog # MAB2698) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. | |
Detection of Human RelB by Western Blot. Western blot shows lysates of Daudi human Burkitts lymphoma cell line and Raji human Burkitts lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human RelB Monoclonal Antibody (Catalog # MAB2698) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for RelB at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4. | |
RelB in Raji Human Cell Line. RelB was detected in immersion fixed Raji human Burkitts lymphoma cell line using Mouse Anti-Human RelB Monoclonal Antibody (Catalog # MAB2698) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
