| 货号 | MAB2195-SP |
| 别名 | CA14; Carbonate dehydratase XIV; carbonic anhydrase XIVcarbonic anhydrase 14; carbonic dehydratase; CAXiV; CA-XIV; EC 4.2.1.1 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunoprecipitation(25 µg/mL) |
| 目标/特异性 | Detects human Carbonic Anhydrase XIV/CA14 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross‑reactivity with recombinant mouse CA14, 10% cross‑reactivity with recombinant human (rh) CA5A, and no cross-reactivity with rhCA1, 2, 3, 4, 6, 7, 8, 9, 10, 12, or 13 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunoprecipitation: 25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 23632 (Human); 23831 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase XIV/CA14 Gly19-Met290 Accession # Q9ULX7 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Carbonic Anhydrase 14 (CA14) has a C-terminal transmembrane domain (aa 291 - 311) and a short cytoplasmic tail (aa 312 - 337). As a functioning enzyme of the CA family, it catalyzes the reversible hydration of CO2. CA14 is highly expressed in all parts of the central nervous system with lower expression in adult liver, heart, small intestine, colon, kidney, urinary bladder and skeletal muscle. The rhCA14 corresponds to the 272 aa ectodomain that has a predicted molecular mass of approximately 32 kDa and an apparent molecular mass of approximately 43 kDa in SDS-PAGE. The amino acid sequence of the human ectodomain shares 98%, 86%, 84%, and 83% identity with that of chimpanzee, bovine/dog, rat and mouse, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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