货号 | MAB2555-SP |
别名 | CD229 antigen; CD229; cell-surface molecule Ly-9; hly9; Ly9; lymphocyte antigen 9Cell surface molecule Ly-9; mLY9; SLAMF3; T-lymphocyte surface antigen Ly-9 | 全称 | SLAM Family Member 3 |
反应种属 | Mouse |
应用 | Western Blot(1 µg/mL) Flow Cytometry(2.5 µg/106cells) |
目标/特异性 | Detects mouse CD229/SLAMF3 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human CD229 is observed. |
使用方法 | Western Blot: 1 µg/mL Flow Cytometry: 2.5 µg/106cells |
来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 4063 (Human); 17085 (Mouse) |
纯化方式 | Protein A or G purified from hybridoma culture supernatant |
免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse CD229/SLAMF3 Lys48-Phe454 Accession # AAH95921 |
生物活性 | Mouse |
标记 | Unconjugated |
溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
背景 | CD229, also known as T lymphocyte surface antigen Ly-9, is a type I transmembrane protein belonging to the immunoglobulin superfamily. It is also a member of the CD150/SLAM receptor family and is expressed on T and B lymphocytes. Mouse CD229 contains 2 Ig-like C2-type domains and 2 Ig-like V-type domains in its extracellular region. Two mouse alleles that differ in 6 extracellular amino acid residues have been reported. Human and mouse CD229 share 60% amino acid sequence identity in their extracellular regions. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Detection of CD229/SLAMF3 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD229/SLAMF3 Monoclonal Antibody (Catalog # MAB2555, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab)2 Secondary Antibody (Catalog # F0105B). |