| 货号 | MAB2199-SP |
| 别名 | AEP; Asparaginyl Endopeptidase; cysteine protease 1; legumain; LGMN; LGMN1; Protease, cysteine 1; protease, cysteine, 1 (legumain); PRSC1EC 3.4.22.34 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunoprecipitation(25 µg/mL) |
| 目标/特异性 | Detects human Legumain/Asparaginyl Endopeptidase (aa18‑433) in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse Legumain is observed. Recognizes the pro form of human Legumain and does not recognize the mature form (aa 18‑323). |
| 使用方法 | Western Blot: 1 µg/mL Immunoprecipitation: 25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5641 (Human); 19141 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Novel candidate genes in unstable areas of human atherosclerotic plaques. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | NS0-derived recombinant human Legumain/Asparaginyl Endopeptidase Ile18-Tyr433 Accession # Q99538 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Legumain is a lysosomal cysteine protease whose activity is found in several tissues tested (1, 2). Legumain plays a pivotal role in the endosomal/lysosomal degradation system because the Legumain deficiency causes the accumulation of pro cathepsins B, H and L, another group of lysosomal cysteine proteases (3). Over-expression of Legumain in tumors is significant for invasion/metastasis (4). Also known as Asparaginyl Endopeptidase, it specifically cleaves peptide bonds with Asn at the P1 position. Nevertheless, it also cleaves peptide bonds with Asp at the P1 position. Auto-activation of pro Legumain involves both types of the cleavage, which result in the removal of the pro peptides in both C- and N-termini (5). In addition, Legumain activates pro MMP-2 and processes bacterial antigens for MHC class II presentation and pro thymosin alpha to thymosin alpha 1 and thymosin alpha 11, two acidic peptides with immunoregulatory properties (6‑8). Human Legumain is synthesized as a 433 amino acid precursor with a signal peptide (residues 1‑17) and a pro enzyme (residues 18‑433). The activity of Legumain can be inhibited by Cystatins C and E/M. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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