| 货号 | MAB3077-SP |
| 别名 | EFABP; E-FABP; E-FABPPAFABP; fatty acid binding protein 5 (psoriasis-associated); Fatty acid-binding protein 5; PA-FABPepidermal; Psoriasis-associated fatty acid-binding protein homolog | 全称 | Fatty Acid-Binding Protein 5 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Flow Cytometry(2.5 µg/106cells) Immunocytochemistry(8-25 µg/mL) |
| 目标/特异性 | Detects human FABP5/E‑FABP in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human FABP1, 2, 3, 4, 6, 7, or 9 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Flow Cytometry: 0.25 µg/106cells Immunocytochemistry: 8-25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2171 (Human); 16592 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Molecular determinants of retinoic acid sensitivity in pancreatic cancer. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human FABP5/E‑FABP Ala2-Glu135 Accession # Q01469 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | FABP5, also known as epidermal fatty acid binding protein (E-FABP), is expressed in skin, lens, adipose tissue, endothelial cells, heart, brain and placenta. FABP-5 is associated with keratinocytes andadipocytes, and is suggested to promote fatty acid availability to enzymes,protect cell structures from fatty acid attack, and target fatty acids tonuclear transcription factors. HumanFABP-5 shares 80%, 81%, and 92% aa identity with mouse, rat and bovine FABP-5,respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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FABP5 in HUVEC Human Cells. FABP5 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Rat Anti-Human FABP5 Monoclonal Antibody (Catalog # MAB3077) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
