Human Carbonic Anhydrase IV MAb (Clone 310413) (25 UG)【科瑞奥博】

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Human Carbonic Anhydrase IV MAb (Clone 310413) (25 UG)

货号： MAB2186-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB2186-SP
别名	CA4; CAIV; CA-IV; Car4; Carbonate dehydratase IV; carbonic anhydrase 4; carbonic anhydrase IVRP17; carbonic dehydratase IV; EC 4.2.1.1; retinitis pigmentosa 17 (autosomal dominant); RP17
反应种属	Human
应用	Western Blot(2 µg/mL) Immunohistochemistry(8-25 µg/mL) Immunoprecipitation(25 µg/mL)
目标/特异性	Detects human Carbonic Anhydrase IV/CA4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 10% cross‑reactivity with recombinant human (rh) CA1, rhCA2, and recombinant mouse CA4 is observed. Does not cross-react with rhCA3, 8, 9, 10, 12, 13, or 14.
使用方法	Western Blot: 2 µg/mL Immunohistochemistry: 8-25 µg/mL Immunoprecipitation: 25 µg/mL
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	762 (Human); 12351 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Transport activity of the high-affinity monocarboxylate transporter MCT2 is enhanced by extracellular carbonic anhydrase IV but not by intracellular carbonic anhydrase II. Authors: Klier M, Schuler C, Halestrap AP, Sly WS, Deitmer JW, Becker HM J. Biol. Chem., 2011;286(31):27781-91. Species: Human Sample Type: Cell Lysates Application: WB Studies on bicarbonate transporters and carbonic anhydrase in porcine nonpigmented ciliary epithelium. Authors: Shahidullah M, To CH, Pelis RM, Delamere NA Invest. Ophthalmol. Vis. Sci., 2009;50(4):1791-800. Species: Porcine Sample Type: Whole Cells Application: ICC
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase IV/CA4 Ala19-Lys283 Accession # P22748
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO₂ + H₂O = HCO₃^- + H⁺, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6^th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO₂ removal and mosquito control in industry. CA4 is a GPI‑anchored membrane enzyme expressed on the luminal surfaces of pulmonary (and certain other) capillaries and of proximal renal tubules. It functions as the principal CO₂ taste sensor (2). In addition, a genetic mutation (Arg 14 to Trp in the signal peptide) of CA4 was found to co‑segregate with the RP17 form of retinitis pigmentosa in two large families and was not found in 36 unaffected family members or 100 controls (3).
运输条件	Blue Ice
存放说明	4℃
参考文献	Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50. Chandrashekar, J. et al. (2009) Science 326:443. Rebello, G. et al. (2004) Proc. Natl. Acad. Sci. USA 101:6617.

参考图片

Detection of Human Carbonic Anhydrase IV/CA4 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and human lung tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Carbonic Anhydrase IV/CA4 Monoclonal Antibody (Catalog # MAB2186) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Carbonic Anhydrase IV/CA4 at approximately 35 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.