Human Carbonic Anhydrase VIII MAb (Clone 308320) (25 UG)【科瑞奥博】

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Human Carbonic Anhydrase VIII MAb (Clone 308320) (25 UG)

货号： MAB2187-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB2187-SP
别名	CA8; CALS; CALScarbonate dehydratase; CAMRQ3; carbonic anhydrase VIIIMGC99509; carbonic anhydrase-like sequence; carbonic anhydrase-related protein; CARP; CARPCA-related protein; CA-VIII; MGC120502
反应种属	Human
应用	Western Blot(1 µg/mL)
目标/特异性	Detects human Carbonic Anhydrase VIII/CA8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CA1, 2, 3, 4, 9, 10, 12, 13, or 14 is observed.
使用方法	Western Blot: 1 µg/mL
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	767 (Human)
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human Carbonic Anhydrase VIII/CA8 Ala2-Gln290 Accession # P35219
生物活性	Human, Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Carbonic Anhydrase VIII (CA8), also called CA‑related protein (CARP), is a cytosolic protein without CA activity (i.e., the reversible hydration of CO₂) due to point mutations in the zinc-binding site (1, 2). Nevertheless, it has esterase activity. CA8 is expressed exclusively in Purkinje cells of the cerebellum, where it binds inositol 1,4,5-triphosphate receptor type 1 (3). CA8 overexpression in human colorectal cancer and non-small cell lung cancer indicates that it plays a role in the process of invasion in these types of malignancy (4, 5). The 289 amino acid rhCA8 has a predicted molecular mass of approximately 33 kDa. It shares 98% amino acid identity with mouse, rat and bovine CA8 and 78% with Xenopus.
运输条件	Blue Ice
存放说明	4℃
参考文献	Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50. Sjoblom, J. et al. (1996) FEBS Lett. 398: 322. Hirota, J. et al. (2003) Biochem. J. 372: 435. Miyaji, E. et al. (2003) J. Pathol. 201: 37. Lu, S.H. et al. (2004) Lung Cancer 44: 273.

参考图片

Detection of Human and Mouse Carbonic Anhydrase VIII/CA8 by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue and mouse brain (cerebellum) tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse Carbonic Anhydrase VIII/CA8 Monoclonal Antibody (Catalog # MAB2187) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Carbonic Anhydrase VIII/CA8 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.