Mouse/Rat Nestin MAb (Clone 307501) (25 UG)【科瑞奥博】

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Mouse/Rat Nestin MAb (Clone 307501) (25 UG)

货号： MAB2736-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB2736-SP
别名	FLJ21841; NES; nestin
反应种属	Mouse/Rat
应用	Western Blot(1 µg/mL) Immunocytochemistry(8-25 µg/mL)
目标/特异性	Detects mouse and rat Nestin in Western blots.
使用方法	Western Blot: 1 µg/mL Immunocytochemistry: 8-25 µg/mL
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	10763 (Human); 18008 (Mouse); 25491 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The PD-1/B7-H1 pathway modulates the natural killer cells versus mouse glioma stem cells. Authors: Huang B, Zhan Y, Zong W, Yu C, Li J, Qu Y, Han S PLoS ONE, 2015;10(8):e0134715. Species: Mouse Sample Type: Whole Cells Application: ICC Nestin delineates pancreatic cancer stem cells in metastatic foci of NOD/Shi-scid IL2Rgamma(null) (NOG) mice. Authors: Matsuda Y, Yoshimura H, Ueda J, Naito Z, Korc M, Ishiwata T Am J Pathol, 2014;184(3):674-85. Species: Mouse Sample Type: Whole Cells Application: Flow VEGF-mediated angiogenesis stimulates neural stem cell proliferation and differentiation in the premature brain. Authors: Sun J, Sha B, Zhou W, Yang Y Biochem. Biophys. Res. Commun., 2010;394(1):146-52. Species: Rat Sample Type: Tissue Homogenates Application: WB
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant rat Nestin Met544-Glu820 (Gly756Asp, Ile758Met, Arg572Lys, Ala574Pro, Ile802Met, Arg816Lys) Accession # EDM00749
生物活性	Mouse, Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Nestin is a class VI intermediate filament protein (1,2) that is expressed in stem cells of the central nervous system (CNS) (3) but not in mature CNS cells.(4) Nestin expression is used extensively as a marker for CNS stem cells in the developing nervous system and in vitro cultured cells (5-10). Its transient expression is a critical step in the neural differentiation pathway.(2) Nestin is also expressed in non-neural stem cell populations, such as pancreatic islet progenitors (11-13) and hematopoietic progenitors (14).
运输条件	Blue Ice
存放说明	4℃
参考文献	Hockfield, S. and R.D. McKay (1985) J. Neurosci. 5:3310. Lendahl, U. et al. (1990) Cell 60:585. Frederiksen, K. and R.D. McKay (1988) J. Neurosci. 8:1144. Tohyama, T. et al. (1992) Lab. Invest. 66:303. Uchida, N. et al. (2000) Proc. Natl. Acad. Sci. USA 97:14720. Frederiksen, K. et al. (1988) Neuron 1:439. Cattaneo, E. et al. (1990) Nature 347:762. Reynolds, B.A. and S. Weiss (1992) Science 255:1707. Rietze, R.L. et al. (2001) Nature 412:736. Carpenter, M.K. et al. (2001) Exp. Neurol. 172:383. Zulewski, H. et al. (2001) Diabetes 50:521. Lumelsky, N. et al. (2001) Science 292:1389. Lechner, A. et al. (2001) Biochem. Biophys. Res. Commun. 293:670. Shih, C.C. et al. (2001) Blood 98:2412.

参考图片

Nestin in Rat Cortical Stem Cells. Nestin was detected in immersion fixed undifferentiated rat cortical stem cells using Mouse Anti‑Rat Nestin Monoclonal Antibody (Catalog # MAB2736) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.