Human IL-22 R alpha 1 MAb (Clone 305405) (25 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human IL-22 R alpha 1 MAb (Clone 305405) (25 UG)

货号： MAB2770-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB2770-SP
别名	CRF2-9; CRF2-9interleukin 22 receptor; Cytokine receptor class-II member 9; Cytokine receptor family 2 member 9; IL-22 receptor subunit alpha-1; IL22R; IL22R1; IL22RA1; IL-22RA1; IL-22R-alpha-1; IL-TIF-R1; interleukin 22 receptor, alpha 1; interleukin-22 receptor subunit alpha-1; zcytoR11
全称	Interleukin 22 Receptor
反应种属	Human
应用	Flow Cytometry(2.5 µg/10⁶cells) Immunocytochemistry(8-25 µg/mL)
目标/特异性	Detects human IL‑22 R alpha 1 in direct ELISAs. In direct ELISAs, no cross‑reactivity with recombinant human (rh) IL-10, rhIL-22BP or rhIL-20 R alpha is observed.
使用方法	Flow Cytometry: 2.5 µg/10⁶cells Immunocytochemistry: 8-25 µg/mL
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	58985 (Human); 230828 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Interleukin-22 suppresses the growth of A498 renal cell carcinoma cells via regulation of STAT1 pathway. Authors: Zhang F, Shang D, Zhang Y, Tian Y PLoS ONE, 2011;6(5):e20382. Species: Human Sample Type: Cell Lysates Application: WB TH17 cytokines induce human airway smooth muscle cell migration. Authors: Chang Y, Al-Alwan L, Risse PA, Roussel L, Rousseau S, Halayko AJ, Martin JG, Hamid Q, Eidelman DH J. Allergy Clin. Immunol., 2011;127(4):1046-53.e1-2. Species: Human Sample Type: Whole Cells Application: Neut Novel mechanism of MDA-7/IL-24 cancer-specific apoptosis through SARI induction. Authors: Dash R, Bhoopathi P, Das S, Sarkar S, Emdad L, Dasgupta S, Sarkar D, Fisher P Cancer Res, 0;74(2):563-74. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Chinese hamster ovary cell line CHO-derived recombinant human IL-22 R alpha 1 Pro18-Thr228 Accession # Q8N6P7
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	IL-22 receptor, also known as IL-22 R alpha 1 and CRF2-9, is an approximately 65 kDa transmembrane glycoprotein in the type II cytokine receptor family (CRF). IL‑22 R alpha 1 contains a 211 amino acid (aa) extracellular domain (ECD) with two fibronectin type III repeats, and a 323 aa cytoplasmic domain. IL-22 R alpha 1 associates with either IL-10 R beta or IL-20 R beta to form receptor complexes with distinct ligand selectivities. IL-10 R beta is a shared subunit of the IL-10, -22, -26, -28, and -29 receptors, while IL-20 R beta is a shared subunit of the IL-19, -20, -22R and -24 receptors (1). IL-22 R alpha 1/IL-10 R beta is an IL-22 responsive receptor (2, 3), and IL-22 R alpha 1/IL-20 R beta is an IL-20 or IL-24 responsive receptor (4, 5). IL-22 R alpha 1 contains cytoplasmic motifs for interactions with signal transduction molecules, but formation of ternary complexes with IL-10 R beta or IL-20 R beta and the respective ligands is required for signal transduction (2, 6). IL-22BP functions as a competitive antagonist by binding IL‑22 and preventing its association with IL-22 R alpha 1 (7, 9). Even though it is a receptor for interleukins, IL-22 R alpha 1 is not expressed on hematopoietic cells (6, 10, 11). Instead, IL-22 R alpha 1 expression is restricted to epithelial and stromal cells (6, 10‑13). IL-22 R alpha 1 signaling promotes innate immune responses and wound healing at sites of infection and inflammation. This includes upregulation of antimicrobial, acute phase, proinflammatory, and extracellular matrix proteins as well as proteases (3, 11, 13, 14). IL-22 R alpha 1 signaling also promotes downregulation of proteins involved in keratinocyte differentiation (3, 14). Within the ECD, human IL-22 R alpha 1 shares 78%, 76%, and 83% aa sequence identity with mouse, rat, and canine IL-22 R, respectively. It shares 22%‑25% aa sequence identity with the ECDs of other class II receptors IL-10 R, IL-20 R, and IL-28 R.
运输条件	Blue Ice
存放说明	4℃
参考文献	Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33. Xie, M.-H. et al. (2000) J. Biol. Chem. 275:31335. Boniface, K. et al. (2005) J. Immunol. 174:3695. Dumoutier, L. et al. (2001) J. Immunol. 167:3545. Wang, M. et al. (2002) J. Biol. Chem. 277:7341. Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725. Li, J. et al. (2004) Int. Immunopharmacol. 4:693. Logsdon, N.J. et al. (2002) J. Interferon Cytokine Res. 22:1099. Kotenko, S.V. et al. (2001) J. Immunol. 166:7096. Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:577. Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:679. Aggarwal, S. et al. (2001) J. Interferon Cytokine Res. 21:1047. Wolk, K. et al. (2004) Immunity 21:241. Wolk, K. et al. (2006) Eur. J. Immunol. 36:1309.

参考图片

Detection of IL‑22 R alpha 1 in COLO 205 Human Cell Line by Flow Cytometry. COLO 205 human colorectal adenocarcinoma cell line was stained with Mouse Anti-Human IL‑22 R alpha 1 Monoclonal Antibody (Catalog # MAB2770, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

IL‑22 R alpha 1 in HT‑29 Human Cell Line.
IL‑22 R alpha 1 was detected in immersion fixed HT‑29 human colon adenocarcinoma cell line using Mouse Anti-Human IL‑22 R alpha 1 Monoclonal Antibody (Catalog # MAB2770) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.