| 货号 | MAB1604-SP |
| 别名 | MAP kinase kinase 6; MAP2K6; MAPK/ERK kinase 6; MAPKK6dual specificity mitogen-activated protein kinase kinase 6; MEK6; MEK6MAPKK 6; mitogen-activated protein kinase kinase 6; MKK6MEK 6; PRKMK6; PRKMK6protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6); SAPKK3; SAPKK3EC 2.7.12.2 | 全称 | Mitogen-activated Protein Kinase Kinase 6 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(0.5 µg/mL) Immunocytochemistry(8-25 µg/mL) |
| 目标/特异性 | Detects human, mouse, and rat MKK6 in Western blots. In Western blots, no cross-reactivity with recombinant human MKK3 is observed. |
| 使用方法 | Western Blot: 0.5 µg/mL Immunocytochemistry: 8-25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5608 (Human); 26399 (Mouse); 114495 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human MKK6 Met1-Asp334 Accession # P52564.1 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | MKK6 and the closely related MKK3 are activated by pro-inflammatory cytokines and environmental stresses. Both MKK3 and MKK6 are dual specificity protein kinases, phosphorylating and activating the p38 MAP kinases at Thr and Tyr positions within the phosphoacceptor sequence Thr-Gly-Tyr. Activation of MKK6 occurs through phosphorylation at Ser207 and Thr211 by several upstream MAPK kinase kinases, including ASK1 and TAK1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human, Mouse, and Rat MKK6 by Western Blot. Western blot shows lysates of B16-F1 mouse melanoma cell line and NRK rat normal kidney cell. PVDF membrane was probed with 0.5 µg/mL Rat Anti-Human/Mouse/Rat MKK6 Monoclonal Antibody (Catalog # MAB1604) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, recombinant human MKK3 and MKK6 (2 ng/lane) were included. A specific band for MKK6 was detected at approximately ??? kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
