Human Carbonic Anhydrase IX MAb (Clone 303104) (25 UG)【科瑞奥博】

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Human Carbonic Anhydrase IX MAb (Clone 303104) (25 UG)

货号： MAB21881-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB21881-SP
别名	CA9; CA-IX; CAIXcarbonic anhydrase 9; Carbonate dehydratase IX; carbonic anhydrase IXpMW1; carbonic dehydratase; EC 4.2.1.1; G250; Membrane antigen MN; MN; MNRCC-associated antigen G250; P54/58N; RCC; RCC-associated protein G250; Renal cell carcinoma-associated antigen G250
反应种属	Human
应用	Immunohistochemistry(8-25 µg/mL)
目标/特异性	Detects human Carbonic Anhydrase IX/CA9 in direct ELISAs.
使用方法	Immunohistochemistry: 8-25 µg/mL
来源	Sterile PBS to a final concentration of 0.5 mg/mL.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	768 (Human); 230099 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Hypoxia-induced Jagged2 promotes breast cancer metastasis and self-renewal of cancer stem-like cells. Authors: Xing F, Okuda H, Watabe M, Kobayashi A, Pai SK, Liu W, Pandey PR, Fukuda K, Hirota S, Sugai T, Wakabayshi G, Koeda K, Kashiwaba M, Suzuki K, Chiba T, Endo M, Mo YY, Watabe K Oncogene, 2011;30(39):4075-86. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase IX/CA9 Pro59-Asp414 Accession # Q16790
生物活性	Human
标记	Unconjugated
溶解方法	Sterile PBS to a final concentration of 0.5 mg/mL.
背景	Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO₂ + H₂O = HCO₃^- + H⁺, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1‑3). CA9, also known as membrane antigen MN and renal cell carcinoma (RCC)‑associated antigen G250, is a transmembrane enzyme expressed primarily in carcinoma cells. It is one of the best markers for hypoxia and for RCC (4, 5). The immunogen corresponds to the extracellular portion of human CA9.
运输条件	Blue Ice
存放说明	4℃
参考文献	Pastorek, J. et al. (1994) Oncogene 9: 2877. Opavsky, R. et al. (1996) Genomics 33: 480. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50. Kaluzova, M. et al. (2004) Mol. Cell Biol. 24:5757. Mukouyama, H. et al. (2004) Clin. Cancer Res. 10:1421.

参考图片

Carbonic Anhydrase IX/CA9 in Human Colon. Carbonic Anhydrase IX/CA9 was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human Carbonic Anhydrase IX/CA9 Monoclonal Antibody (Catalog # MAB21881) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.