Human Akt2 MAb (Clone 302501) (25 UG)【科瑞奥博】

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Human Akt2 MAb (Clone 302501) (25 UG)

货号： MAB23152-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB23152-SP
别名	EC 2.7.11; EC 2.7.11.1; Murine thymoma viral (v-akt) homolog-2; PKB beta; PKBB; PKBBETA; PRKBB; Protein kinase Akt-2; Protein kinase B beta; rac protein kinase beta; RAC-beta serine/threonine-protein kinase; RAC-beta; RAC-PK-beta; v-akt murine thymoma viral oncogene homolog 2
全称	v-Akt Murine Thymoma Viral Oncogene Homolog 2
反应种属	Human
应用	Immunohistochemistry(8-25 µg/mL) Intracellular Staining by Flow Cytometry(2.5 µg/10⁶cells)
目标/特异性	Detects human Akt2. Using direct ELISA, this antibody does not detect recombinant human (rh) Akt1 or rhAkt3.
使用方法	Immunohistochemistry: 8-25 µg/mL Intracellular Staining by Flow Cytometry: 2.5 µg/10⁶cells
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	208 (Human); 11652 (Mouse); 29414 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Molecular profiling of cervical cancer progression. Authors: Hagemann T, Bozanovic T, Hooper S, Ljubic A, Slettenaar VI, Wilson JL, Singh N, Gayther SA, Shepherd JH, Van Trappen PO Br. J. Cancer, 2007;96(2):321-8. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Akt2 Asn2-Glu481 Accession # P31751
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	The serine/threonine kinase Akt, also known as protein kinase B (PKB), is a central regulator of such diverse cellular processes as glucose uptake, cell cycle progression, and apoptosis. In mammals, three highly homologous members define the Akt family: Akt1 (PKB alpha ), Akt2 (PKB beta ), and Akt3 (PKB gamma ). Akt2 is expressed predominantly in insulin target tissues such as liver, skeletal muscle, and fat.
运输条件	Blue Ice
存放说明	4℃
参考文献	A Novel Pathway for TOR-independent Autophagy Regulation by Akt Akt Modulates TGF-beta Activities Apoptosis Intracellular Kinases Chemokine Receptor Intracellular Signaling Common gamma Chain Receptor Family Diabetic Peripheral Neuropathy GDNF Family Human Phospho-MAPK Array Kit ICC / IHC Images: Akt2 Intracellular Kinases Intracellular Kinases in the Akt Pathway Intracellular Signaling Molecules in Angiogenesis Neurotrophin/Trk Family Notch Pathway PDGF Family TNF Superfamily Regulators VEGF Family

参考图片

Detection of Akt2 in MCF‑7 Human Cell Line by Flow Cytometry. MCF‑7 human breast cancer cell line was stained with Mouse Anti-Human Akt2 Monoclonal Antibody (Catalog # MAB23152, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab)₂ Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Akt2 in Human Pancreatic Cancer Tissue. Akt2 was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using 25 µg/mL Mouse Anti-Human Akt2 Monoclonal Antibody (Catalog # MAB23152) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.