Human Akt1 MAb (Clone 302407) (25 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human Akt1 MAb (Clone 302407) (25 UG)

货号： MAB17751-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB17751-SP
别名	AKT; EC 2.7.11; EC 2.7.11.1; PKB alpha; PKBMGC99656; PRKBA; Protein kinase B; Proto-oncogene c-Akt; rac protein kinase alpha; RAC-alpha serine/threonine-protein kinase; RAC-alpha; RAC-PK-alpha; RACPKB-ALPHA; v-akt murine thymoma viral oncogene homolog 1
全称	v-Akt Murine Thymoma Viral Oncogene Homolog 1
反应种属	Human
应用	Immunocytochemistry(8-25 µg/mL) Intracellular Staining by Flow Cytometry(2.5 µg/10⁶cells)
目标/特异性	Detects human Akt1 in direct ELISAs. Reactivity with Akt1 from other species has not been determined. In direct ELISAs, no cross-reactivity with recombinant human (rh) Akt2 or rhAkt3 is observed.
使用方法	Immunocytochemistry: 8-25 µg/mL Intracellular Staining by Flow Cytometry: 2.5 µg/10⁶cells
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	207 (Human); 11651 (Mouse); 25233 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Effect of Akt inhibition on scatter factor-regulated gene expression in DU-145 human prostate cancer cells. Authors: Xu J, Gao M, Fan S, Meng Q, Goldberg ID, Abounader R, Ressom H, Laterra JJ, Rosen EM Oncogene, 2007;26(20):2925-38. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Akt1 Ser2-Ala480 Accession # P31749
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Akt, also known as protein kinase B (PKB), is a central kinase in such diverse cellular processes as glucose uptake, cell cycle progression, and apoptosis. Three highly homologous members define the Akt family: Akt1 (PKB alpha ), Akt2 (PKB beta ), and Akt3 (PKB gamma ). Akt1 is the most ubiquitously expressed family member. All three Akts contain an amino-terminal pleckstrin homology domain, a central kinase domain, and a carboxyl-terminal regulatory domain.
运输条件	Blue Ice
存放说明	4℃
参考文献	A Novel Pathway for TOR-independent Autophagy Regulation by Akt Akt Modulates TGF-beta Activities Apoptosis Intracellular Kinases Chemokine Receptor Intracellular Signaling Common gamma Chain Receptor Family Diabetic Peripheral Neuropathy GDNF Family Growth Hormone/IGF-I Axis Human Phospho-MAPK Array Kit ICC / IHC Images: Akt1 Intracellular Kinases Intracellular Kinases in the Akt Pathway Intracellular Signaling Molecules in Angiogenesis Neurotrophin/Trk Family Notch Pathway PDGF Family TNF Superfamily Regulators VEGF Family

参考图片

Detection of Akt1 in MCF‑7 Human Cell Line by Flow Cytometry. MCF‑7 human breast cancer cell line was stained with Mouse Anti-Human Akt1 Monoclonal Antibody (Catalog # MAB17751, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab)₂ Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.