| 货号 | MAB2855-SP |
| 别名 | ERK activator kinase 2; MAP2K2; MAPKK 2; MEK 2; MEK2EC 2.7.12.2; mitogen-activated protein kinase kinase 2; MKK2; MKK2FLJ26075; p45; PRKMK2MAPKK2 | 全称 | Mitogen-activated Protein Kinase Kinase 2 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(0.5 µg/mL) Immunocytochemistry(8-25 µg/mL) |
| 目标/特异性 | Detects human, mouse, and rat MEK2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) MEK1 or rhMEK5 is observed. |
| 使用方法 | Western Blot: 0.5 µg/mL Immunocytochemistry: 8-25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5605 (Human); 26396 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human MEK2 Met1-Val400 Accession # P36507 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | MEK1 and MEK2, also known as MAP2K1 and MAP2K2, are activated in response to mitogenic stimuli. Activation occurs through dual phosphorylation at S218/S222 of MEK1 and S222/S226 of MEK2 by several upstream MAP3Ks. Both MEK1 and MEK2 are dual-specificity protein kinases, phosphorylating and activating ERK1 and ERK2 at Thr and Tyr positions. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human/Mouse/Rat MEK2 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, U937 human histiocytic lymphoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/Mouse/Rat MEK2 Monoclonal Antibody (Catalog # MAB2855) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human MEK1 and Recombinant Human Active MEK2 (Catalog # 2855-KS) (5 ng/lane) were included. A specific band for MEK2 was detected at approximately ??? kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3. |
