| 货号 | MAB2558-SP |
| 别名 | 9.5S alpha-1-glycoprotein; amyloid P component, serum; APCS; MGC88159; PTX2; PTX2serum amyloid P-component; SAP; SAPpentaxin-related | 全称 | Serum Amyloid P Component |
| 反应种属 | Mouse |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects mouse Pentraxin 2/SAP in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross‑reactivity with recombinant rat Pentraxin 2/SAP is observed. In Western blots, 100% cross-reactivity with recombinant human (rh) Pentraxin 2/SAP is observed, but no cross-reactivity with rhPentraxin 2/SAP in direct ELISAs is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 325 (Human); 20219 (Mouse); 29339 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse Pentraxin 2/SAP Gln21-Asp224 Accession # P12246 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Pentraxin 2 (also known as Serum Amyoid P Component or SAP) is a secreted glycoprotein that is a universal non-fibrillar component of amyloid deposits. Amyloid is an abnormal extracellular deposit of insoluble protein fibrils that can lead to tissue damage and disease (1‑3). Pentraxin 2 belongs to the pentraxin (pentaxin) family, whose members have a characteristic pentagonal discoid arrangement of five non-covalently bound subunits (4). Pentraxin domains contain the consensus sequence, HxCx(S/T)WxS (x = any amino acid), a lectin fold, and two calcium-binding sites (1). They bind to a variety of unrelated molecules in a calcium-dependent lectin-like manner (1, 4, 5). Pentraxin 2 and C-reactive protein (CRP) are members of the classical or short pentraxin subfamily and share 46% amino acid (aa) identity (1). Mouse Pentraxin 2 is the major acute-phase protein whose expression is dependent on complement activation, IL-6 and/or IL-1 beta, while in humans, CRP is the major acute-phase protein (2, 5, 9). Both are produced and secreted by liver hepatocytes and circulate in plasma. The 204 aa mature mouse Pentraxin 2 shares 79% aa identity with rat Pentraxin 2 and 63‑68% aa identity with human, guinea pig, golden hamster, porcine, and bovine Pentraxin 2 (2, 5). Amyloid deposits containing Pentraxin 2 are implicated in a diverse range of diseases including Alzheimer’s, prion diseases, type 2 diabetes and various systemic amyloidoses (3, 6, 7). Pentraxin 2 regulates the solubility of amyloid fibrils and protects them from degradation. In addition to its pathogenic role, Pentraxin 2 also has an important physiological function in innate immunity (8). It is an opsonin that interacts with all three types of human Fc gamma receptors that mediate neutrophil phagocytosis (8). Pentraxin 2 has been proposed to bind and sequester a variety of ligands including auto-antigens, apoptotic cells, chromatin, DNA, and micro-organisms (1‑3). Pentraxin 2 is also a normal component of basement membranes (1). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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