Human/Equine Cytochrome c MAb (Clone 2B5F8) (25 UG)【科瑞奥博】

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Human/Equine Cytochrome c MAb (Clone 2B5F8) (25 UG)

货号： MAB898-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB898-SP
别名	CYCHCS; CYCS; cytochrome c; cytochrome c, somatic; THC4
反应种属	Human/Equine
应用	Immunohistochemistry(8-25 µg/mL) Immunoprecipitation(1 µg/10⁶cells) Immunocytochemistry(8-25 µg/mL)
目标/特异性	Detects native human Cytochrome c but not mouse Cytochrome c.
使用方法	Immunohistochemistry: 8-25 µg/mL Immunoprecipitation: 1 µg/10⁶cells Immunocytochemistry: 8-25 µg/mL
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	54205 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Ellipticine derivative NSC 338258 represents a potential new antineoplastic agent for the treatment of multiple myeloma. Authors: Tian E, Landowski TH, Stephens OW, Yaccoby S, Barlogie B, Shaughnessy JD Mol. Cancer Ther., 2008;7(3):500-9. Species: Human Sample Type: Whole Cells Application: ICC
纯化方式	Protein A or G purified from ascites
免疫原	Equine Cytochrome c
生物活性	Human, Equine
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Cytochrome c is a mitochondrial electron transport protein thatfunctions in oxidative phosphorylation. Release of Cytochrome c is anearly step in apoptotic cell death. Human and equine Cytochrome c share 89% amino acid sequence identity.
运输条件	Blue Ice
存放说明	4℃
参考文献	ALS Related Molecules Bcl-2 Proteins and Regulators ICC / IHC Images: Cytochrome c Mini-review: Apoptosis I Mini-review: Apoptosis II: Beyond Cytochrome c; the Other Mitochondrial Proteins Mitochondrial Proteins Recombinant Human Bcl-xL Cytochrome c Release Assay Recombinant Human BID Cytochrome c Release Assay Recombinant Human Caspase-8-cleaved BID Cytochrome c Release Assay Recombinant Mouse BID Cytochrome c Release Assay Recombinant Mouse Caspase-8-cleaved BID Cytochrome c Release Assay

参考图片

Immunoprecipitation of Human/Mouse Cytochrome c. Cytochrome c was immunoprecipitated from lysates (5 x 10⁶ cells) of Jurkat human acute T cell leukemia and CTLL‑2 mouse cytotoxic T cell line following incubation with 5 µg Human/Equine Cytochrome c Monoclonal Antibody (Catalog # MAB898) for 1 hour at 4 ºC. Cytochrome c-antibody complexes were absorbed using Protein A Immunoprecipitin (Life Technologies). Immunoprecipitated Cytochrome c (left panel) was detected by Western blot using 0.5 µg/mL Human/Mouse/Rat Cytochrome c Monoclonal Antibody (Catalog # MAB897). For additional reference, Western blot (right panel) shows lysates of Jurkat and CTLL2 cell lines probed with 15 µg/mL Human/Mouse/Rat Cytochrome c Monoclonal Antibody (Catalog # MAB897). View our recommended buffer recipes for immunoprecipitation.

Cytochrome c in Human PBMCs. Cytochrome c was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with ionomycin and PMA using Human/Equine Cytochrome c Monoclonal Antibody (Catalog # MAB898) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.