Human/Mouse Sulfatase-2/SULF2 MAb (Clone 2B4) (25 UG)
货号：
MAB7087-SP 基本售价：
1467.3 元 规格：
-
产品信息
概述| 货号 | MAB7087-SP |
| 别名 | DKFZp313E091; EC 3.1.6; EC 3.1.6.-; EC 3.1.6.14; extracellular sulfatase Sulf-2; FLJ44488; FLJ90554; hSulf-2; KIAA1247HSULF-2; MGC126411; SULF2; SULF-2; sulfatase 2 |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(1 µg/mL)
|
| 目标/特异性 | Detects human Sulfatase-2/SULF2 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL
|
| 来源 | Sterile PBS to a final concentration of 0.5 mg/mL. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 55959 (Human); 72043 (Mouse); 311642 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Recombinant human Sulfatase-2/SULF2, used to immunize a SULF2-deficient mouse
Accession # NP_940998 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.5 mg/mL. |
| 背景 | Sulfatase 2 (SULF2) is a 132 kDa calcium-bindingglycoprotein that is one of two extracellular sulfatases (SULFs). SULFs degradecell surface and extracellular matrix heparin sulfate proteoglycans, alteringthe binding and signaling of factors such as Wnts and FGFs. Dysregulation ofSULF2 is implicated in carcinogenesis. A high molecular weight form (250 kDa)is presumed to be a covalent dimer, while smaller forms (60 kDa and 64 kDa) areproduced by proteolytic cleavage. A splicing form, isoform b, differs only in non-coding regions. Within the region used as an immunogen, humanSULF2 shares approximately 94% amino acid sequence identity with mouse and ratSULF2, while mouse and rat SULF2 share 98% amino acid sequence identity with each other. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - Lemjabbar-Alaoui, H. et al. (2010) Oncogene 29:635.
|
参考图片Detection of Human Sulfatase‑2/SULF2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse Sulfatase‑2/SULF2 Monoclonal Antibody (Catalog # MAB7087) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Sulfatase‑2/SULF2 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
