| 货号 | MAB3228-SP |
| 别名 | CBP; eIF4E; eIF-4E; EIF4E1; EIF4EL1; EIF4EL1MGC111573; eIF-4F 25 kDa subunit; EIF4F; eukaryotic translation initiation factor 4E; eukaryotic translation initiation factor 4E-like 1; mRNA cap-binding protein | 全称 | Eukaryotic Translation Initiation Factor 4E |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(0.1 µg/mL) Immunocytochemistry(8-25 µg/mL) |
| 目标/特异性 | Detects human, mouse and rat eIF4E. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunocytochemistry: 8-25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1977 (Human); 13684 (Mouse); 117045 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human eIF4E Met1-Val217 Accession # P06730 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | eIF4E (eukaryotic initiation factor 4E) interacts with the 7-methyl-GTP cap structure to facilitate the initiation and rate of translation of mRNA. Together with eIF4G and eIF4A, it forms the eIF4F complex. eIF4E activity has been shown to play a role in cell cycle progression, tumorigenesis, embryonic development, nuclear export and synaptic plasticity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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eIF4E in MCF-7 Human Cell Line. eIF4E was detected in immersion fixed MCF-7 human breast cancer cell line using 10 µg/mL Mouse Anti-Human/Mouse/Rat eIF4E Monoclonal Antibody (Catalog # MAB3228) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. | |
Detection of Human/Mouse/Rat eIF4E by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line, Balb/3T3 mouse embryonic fibroblast cell line, and PC‑12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat eIF4E Monoclonal Antibody (Catalog # MAB3228) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for eIF4E at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
