货号 | MAB18561-SP |
别名 | CD2 antigen (p50), sheep red blood cell receptor; CD2 antigen; CD2 molecule; CD2; Erythrocyte receptor; FLJ46032; LFA-2; LFA-3 receptor; lymphocyte-function antigen-2; Rosette receptor; SRBC; T11; T-cell surface antigen CD2; T-cell surface antigen T11/Leu-5 |
反应种属 | Human |
应用 | Flow Cytometry(2.5 µg/106cells) Immunocytochemistry(8-25 µg/mL) |
目标/特异性 | Detects recombinant human CD2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) CD2 or rmCD2F-10 is observed. |
使用方法 | Flow Cytometry: 2.5 µg/106cells Immunocytochemistry: 8-25 µg/mL |
来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 914 (Human); 12481 (Mouse) |
纯化方式 | Protein A or G purified from hybridoma culture supernatant |
免疫原 | Mouse myeloma cell line NS0-derived recombinant human CD2 Lys25-Asp209 Accession # P06729 |
生物活性 | Human |
标记 | Unconjugated |
溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
背景 | CD2 is a type I glycoprotein belonging to the Ig superfamily and isexpressed on T, NK, B, and some antigen presenting cells. Human CD2binds human CD58 on antigen-presenting cells to induce co-stimulatingsignals in T cells. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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CD2 in Human PBMCs. CD2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Mouse Anti-Human CD2 Monoclonal Antibody (Catalog # MAB18561) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Donkey Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |