| 货号 | MAB2048-SP |
| 简介 | Clone 296902 was used by HLDA to establish CD designation |
| 别名 | CD307b antigen; CD307b; Fc receptor homolog 2; Fc receptor-like 2; FcRH2; FCRH2SPAP1B; FcRL2; FcR-like protein 2; IFGP family protein 4; IFGP4; Immunoglobulin receptor translocation-associated protein 4; immunoglobulin superfamily Fc receptor, gp42; IRTA4; IRTA4SPAP1C; SH2 domain containing phosphatase anchor protein 1; SH2 domain-containing phosphatase anchor protein 1Fc receptor-like protein 2; SPAP1; SPAP1A; SPAP1immune receptor translocation-associated protein 4 | 全称 | Fc Receptor-like 2 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Flow Cytometry(2.5 µg/106cells) |
| 目标/特异性 | Detects human FCRL2/FcRH2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) FCRL1, 3, 4, or 5 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Flow Cytometry: 2.5 µg/106cells |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 79368 (Human) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Analysis of HLDA9 mAbs on plasmacytoid dendritic cells. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human FCRL2/FcRH2 Glu15-Asp395 Accession # Q96LA5 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Fc receptor-like 2 (FCRL2), also known as FcRH2 and IRTA4, belongs to the family of glycoprotein homologs of classical immunoglobulin (Ig) Fc receptors. In human, the type I transmembrane FCRL protein family contains from three to nine immunoglobulin-like domains (1, 2). Mature human FcRH2 consists of a 382 amino acid (aa) extracellular domain (ECD) with four Ig-like C2-set domains, a 21 aa transmembrane segment, and an 86 aa cytoplasmic domain with one ITAM-like, and two ITIM-like motifs (3‑5). Alternate splicing of human FCRL2 may generate isoforms with N-terminal, internal, or C-terminal deletions (4, 5). The gene for FcRH2 maps to the human Iq21-23 locus which is a hotspot for chromosomal translocation events associated with B cell malignancies (3, 6). Although there are several Fc receptor-like genes in the mouse, none of these is a clear ortholog to human FCRL2 (7). FCRL proteins are differentially expressed among B cells (2). FCRL2 is preferentially expressed on naïve and CD27+ memory B cells within the spleen, lymph nodes, tonsils, and peripheral blood (3, 4, 8, 9). It is also expressed on most B cells in B cell chronic lymphocytic leukemia (B-CLL) patients (10). FCRL2 upregulation is associated with mutation of the immunoglobulin heavy chain variable (IGHV) and less aggressive forms of B-CLL (9, 11).
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| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of FCRL2/FcRH2 in Human PBMCs by Flow Cytometry. Human peripheral blood monocytes (PBMC) were labeled with Mouse Anti-Human FCRL2/FcRH2 Monoclonal Antibody (Catalog # MAB2048) followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab)2 Secondary Antibody (Catalog # F0101B) and Human CD19 PE-conjugated Monoclonal Antibody (Catalog # FAB4867P). Quadrant markers were set based on control antibody staining (Catalog # MAB002). |
