| 货号 | MAB2410-SP |
| 别名 | CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4 |
| 反应种属 | Canine |
| 应用 | Western Blot(1 µg/mL) Flow Cytometry(2.5 µg/106cells) Immunocytochemistry(15-30 µg/mL) |
| 目标/特异性 | Detects canine CD4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CD4, recombinant mouse CD4, and recombinant feline CD4 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Flow Cytometry: 2.5 µg/106cells Immunocytochemistry: 15-30 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 493775 (Feline) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant canine CD4 Val25-Lys401 Accession # P33705 |
| 生物活性 | Canine |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | CD4 is a type I membrane glycoprotein belonging to the immunoglobulin superfamily. It is expressed predominantly on thymocytes and a subset of mature T lymphocytes. CD4 functions in collaboration with the T cell receptor in the recognition of peptide antigens that are presented by class II major histocompatibility complexes. CD4 also has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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CD4 in Canine PBMCs. CD4 was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Canine CD4 Monoclonal Antibody (Catalog # MAB2410) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
